Title Avian influenza virus / Erica Spackman, editor

Published Totowa, N.J. : Humana Press, ©2008

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Description 1 online resource (x, 141 pages) : illustrations Series Methods in molecular biology ; v. 436 Methods in molecular biology (Clifton, N.J.) ; v. 436 Contents A brief introduction to the avian influenza virus / Erica Spackman -- Avian influenza virus sample types, collection, and handling / Mary Lea Killian -- Avian influenza virus RNA extraction from tissue and swab material / Erica Spackman and David L. Suarez -- Type A influenza virus detection and quantitation by real-time RT-PCR / Erica Spackman and David L. Suarez -- Detection and identification of the H5 hemagglutinin subtype by real-time RT-PCR -- Avian influenza virus isolation and propagation in chicken eggs / Peter R. Woolcock -- Hemagglutination-inhibition test for avian influenza virus subtype identification and the detection and quantitation of serum antibodies to the avian influenza virus / Janice C. Pedersen -- Neuraminidase-inhibition assay for the identification of influenza A virus neuraminidase subtype or neuraminidase antibody specificity / Janice C. Pedersen -- Immunohistochemical staining for the detection of the avian influenza virus in tissues / Mary J. Pantin-Jackwood -- Wild bird surveillance for the avian influenza virus / Justin D. Brown and David E. Stallknecht -- Reverse genetics of the avian influenza virus / Chang-Won Lee and David L. Suarez -- Evaluating the cell-mediated immune response of avian species to avian influenza viruses / Darrell R. Kapczynski -- Measurement of avian cytokines with real-time RT-PCR following infection with the avian influenza virus / Darrell R. Kapczynski and Michael H. Kogut Summary With the growing global fear of a major pandemic, avian influenza virus research has increased greatly in importance during this young century. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental yet essential virological methods for AI virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research. Exciting, cutting-edge new methods focus on studying the virus itself and work with avian hosts, an area greatly lacking in research. Following the format of the highly successful Methods in Molecular Biology series, each chapter provides readily reproducible laboratory protocols offering step-by-step instruction and lists of the necessary equipment for the task. Comprehensive and timely, Avian Influenza Virus equips diagnosticians and researchers with the current tools and information they need to learn more about this high impact disease Analysis aviaire influenzavirussen avian influenza viruses avian influenza a viruses epidemiologie epidemiology volksgezondheid public health medische parasitologie medical parasitology moleculaire virologie molecular virology humane pathogenen human pathogens Epidemiology Human Pathology Epidemiologie Humane pathologie Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Avian influenza -- Research -- Methodology Birds -- Diseases -- Research Virology. Methodology. Microbiology. Biology. Virus diseases. Life sciences. Orthomyxoviruses. RNA viruses. Physical sciences. Diseases. Viruses. Orthomyxoviridae Infections Laboratory Manuals Virology Methods Influenza A virus Genetics Alphainfluenzavirus Publication Formats Investigative Techniques Microbiology Biology RNA Virus Infections Virus Diseases Biological Science Disciplines Publication Characteristics Analytical, Diagnostic and Therapeutic Techniques and Equipment Orthomyxoviridae RNA Viruses Natural Science Disciplines Disease Vertebrate Viruses Viruses Disciplines and Occupations Organisms methodology. microbiology. biology. biological sciences. physical sciences. Biology Diseases Life sciences Methodology Microbiology Orthomyxoviruses Physical sciences RNA viruses Virology Virus diseases Viruses Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Spackman, Erica ISBN 9781597452793 1597452793 1588299392 9781588299390

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