Title Virus-host interactions : methods and protocols / edited by Susanne M. Bailer, Institute for Interfacial Engineering and Plasma Technology, University of Stuttgart, Stuttgart, Germany, Diana Lieber, Institute of Virology, Ulm University Medical Center, Ulm, Germany

Published New York : Humana Press, 2013

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Description 1 online resource (x, 372 pages) : illustrations (some color) Series Methods in molecular biology, 1064-3745 ; 1064 Methods in molecular biology (Clifton, N.J.) ; 1064. 1064-3745 Contents 880-01 High-throughput yeast two-hybrid protocol to determine virus-host protein interactions / Hannah Striebinger, Manfred Koegl, and Susanne M. Bailer -- Analysis of protein-protein interactions using LUMIER assays / Sonja Blasche and Manfred Koegl -- Detection of protein interactions during virus infection by bimolecular fluorescence complementation / Stefan Becker and Jens von Einem -- Discovery of host-viral protein complexes during Infection / Daniell L. Rowles, Scott S. Terhune, and Ileana M. Cristea -- Screening for host proteins with pro- and antiviral activity using high-throughput RNAi / Samantha J. Griffiths -- High resolution gene expression profiling of RNA synthesis, processing, and decay by metabolic labeling of newly transcribed RNA using 4-thiouridine / Lars Dölken -- Isolation and characterization of pathogen-bearing endosomes enable analysis of endosomal escape and identification of new cellular cofactors of infection / Konstanze D. Scheffer, Ruth Popa-Wagner, and Luise Florin -- Computational Analysis of Virus-Host interactomes / Caroline C. Friedel -- Interspecies heterokaryon assay to characterize the nucleocytoplasmic shuttling of herpesviral proteins / Shuai Wang, Kezheng Wang, and Chunfu Zheng -- Detection of integrated herpesvirus genomes by fluorescence in situ hybridization (FISH) / Benedikt B. Kaufer -- Fast generation of stable cell lines expressing fluorescent marker molecules to study pathogen induced processes / Jens Bernhard Bosse, Jessica Ragues, and Harald Wodrich -- Determination of HSV-1 infectivity by plaque assay and a luciferase reporter cell line / Diana Lieber and Susanne M. Bailer -- Generation of a stable cell line for constitutive miRNA expression / Diana Lieber -- Applications for a dual fluorescent human cytomegalovirus in the analysis of viral entry / Kerstin Laib Sampaio, Gerhard Jahn, and Christian Sinzger -- High temporal resolution imaging reveals endosomal membrane penetration and escape of adenoviruses in real time / Ruben Martinez [and others] -- Three-dimensional visualization of virus-infected cells by serial sectioning : an electron microscopic study using resin embedded cells / Martin Schauflinger, Clarissa Villinger, and Paul Walther -- 3D-tissue model for herpes simplex virus-1 infections / Ina Hogk, Steffen Rupp, and Anke Burger-Kentischer -- In vivo visualization of encephalitic lesions in herpes simplex virus type 1 (HSV-1) infected mice by magnetic resonance imaging (MRI) / Wali Hafezi and Verena Hoerr -- Detection of antigen-specific T cells based on intracellular cytokine staining using flow-cytometry / Tina Schmidt and Martina Sester -- Functional NK cell cytotoxicity assays against virus infected cells / Rebecca J. Aicheler and Richard J. Stanton -- Assessment of natural killer cell responses to human cytomegalovirus-infect / Zeguang Wu, Giada Frascaroli, and Thomas Mertens -- Analysis of cell migration during human cytomegalovirus (HCMV) infection / Stefania Varani and Giada Frascaroli -- Gene targeting in mice : a review / Hicham Bouabe and Klaus Okkenhaug -- Protocol for construction of gene targeting vectors and generation of homologous recombinant embryonic stem cells / Hicham Bouabe and Klaus Okkenhaug -- Measurement of mouse cytomegalovirus-induced interferon-[beta] with immortalized luciferase reporter cells / Evgenia Scheibe [and others] 880-01/(S A High-Throughput Yeast Two Hybrid Protocol to Determine Virus-Host Protein Interactions -- Analysis of Protein-Protein Interactions Using LUMIER Assay -- Detection of Protein Interactions During Virus Infection by Bimolecular Fluorescence Complementation -- Discovery of Host-Viral Protein Complexes During Infection -- Screening for Host Proteins with Pro- and Antiviral Activity Using High-Throughput RNAi -- High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay by Metabolic Labelling of Newly Transcribed RNA using 4-thiouridine -- Isolation and Characterization of Pathogen-Bearing Endosomes Enables Identification of New Cellular Cofactors and Analysis of Endosomal Escape -- Computational Analysis of Virus-Host Interactomes -- Interspecies Heterokaryon Assay to Characterize the Nucleocytoplasmic Shuttling of Herpesviral Proteins -- Detection of Integrated Herpesvirus Genomes by Fluorescent in situ Hybridization (FISH) -- Fast Generation of Stable Cell Lines Expressing Fluorescent Marker Molecules to Study Pathogen Induced Processes -- Determination of HSV-1 Infectivity Using a Luciferase Reporter Cell Line -- Generation of a Cell Line for Constitutive Expression of Host or Viral miRNAs -- Applications for a Dual Fluorescent Human Cytomegalovirus in the Analysis of Viral Entry -- High Temporal Resolution Imaging Reveals Endosomal Membrane Penetration and Escape of Adenoviruses in Real-Time -- Three-Dimensional Visualization of Virus Infected Cells by Serial Sectioning -- An Electron Microscopic Study Using Resin Embedded Cells -- 3D Tissue Model for Herpes Simplex Virus-1 Infections -- In vivo Visualization of Encephalitic Lesions in Herpes Simplex Virus Type 1 (HSV-1) Infected Mice by Magnetic Resonance Imaging (MRI) -- Detection of Antigen-Specific T Cells Based on Intracellular Cytokine Staining Using Flow-Cytometry -- Functional NK Cell Cytotoxicity Assays Against Virus Infected Cells -- Assessment of Natural Killer Cell Responses to Human Cytomegalovirus Infected Macrophages -- Analysis of Cell Migration During HCMV Infection -- Gene Targeting in Mice: A Review -- A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant ES Cells -- Measurement of Mouse Cytomegalovirus Induced Interferon-β with Immortalized Luciferase Reporter Cells Summary Virus-Host Interactions: Methods and Protocols covers various aspects of virological research, such as biochemical approaches, including molecular interactions and regulatory mechanisms on the protein as well as the RNA level with a strong focus on the manifold possibilities to study protein-protein interactions, as well as cell biological and immunological methodologies. Viruses represent a reduced form of life that depends on host cells for propagation. To this end, viruses approach and penetrate cells and usurp cellular machineries for their own benefit. Recent technological improvements have enabled the systematic analysis of the virus-host interplay be it on the genomic, the transcriptomic, or proteomic level. In parallel, bioinformatic tools have emerged in support of the large datasets generated by these high-throughput approaches. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Virus-Host Interactions: Methods and Protocols will prove invaluable to professionals and novices with its well-honed methodologies and protocols Analysis virussen viruses host pathogen interactions biomedische wetenschappen biomedicine immunologie immunology bioinformatics laboratoriummethoden laboratory methods protocollen protocols Virology Virologie Notes Includes index Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (SpringerLink, viewed September 3, 2013) Subject Host-virus relationships -- Laboratory manuals Microbiology. Biology. Life sciences. Physical sciences. Viruses. Virology. Methodology. Organisms Investigative Techniques Publication Formats Microbiological Phenomena Microbiology Biological Phenomena Publication Characteristics Biology Analytical, Diagnostic and Therapeutic Techniques and Equipment Phenomena and Processes Biological Science Disciplines Natural Science Disciplines Disciplines and Occupations Viruses Host-Pathogen Interactions Laboratory Manuals Virology Virus Physiological Phenomena Methods microbiology. biology. biological sciences. physical sciences. methodology. Viruses Virology Physical sciences Microbiology Methodology Life sciences Biology Host-virus relationships Genre/Form Laboratory manuals Form Electronic book Author Bailer, Susanne M., editor Lieber, Diana, editor ISBN 9781627036016 1627036016 1627036008 9781627036009

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