Description |
1 online resource (x, 312 pages) : illustrations |
Series |
Springer protocols |
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Methods in molecular biology, 1064-3745 ; 550 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 550.
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Contents |
Human embryogenesis: overview / C. Vaillancourt and J. Lafond -- Differential gene expression in the uterus and blastocyst during the reactivation of embryo development in a model of delayed implantation / P.L. Lefevre and B.D. Murphy -- Coculture of decidua and trophoblast to study proliferation and invasion / M. Cohen and P. Bischof -- Isolation and culture of term human cytotrophoblast cells and in vitro methods for studying human cytotrophoblast cells' calcium uptake / F. Le Bellego, C. Vaillancourt and J. Lafond -- Endocrinology and cell signaling in human villous trophoblast / C. Mounier [and others] -- Gestation-induced uterine vascular remodeling / P.A. Scott [and others] -- Placental and fetal steroidogenesis / J.T. Sanderson -- Current methods in investigating the development of the female reproductive system / P.J. Devine, P.B. Hoyer and A.F. Keating -- A revised and improved method for the isolation of seminiferous tubule-enriched fractions that preserves the phosphorylated and glycosylated forms of proteins / C.D. Akpovi and R. Marc Pelletier -- A novel technical approach for the measurement of individual ACAT-1 and ACAT-2 enzymatic activity in the testis / L. Chen, J. Lafond and R.M. Pelletier -- Genetic dissection of Caenorhabditis elegans embryogenesis using RNA interference and flow cytometry / E.L. Boulier and S. Jenna -- Immune system: maturation of myeloid cells / J. Ennaciri and D. Girard -- Functional development of human fetal gastrointestinal tract / E. Levy [and others] -- Renal and cardiac Na+-K +-ATPase and aconitase in a rat model of fetal programming / R. Gaudet and M. Brochu -- Assessment of embryotoxicity using mouse embryo culture / L.M. Winn and E.W. Tung -- Fertilization in vitro / Y. Bing and R.J. Ouellette -- Animal cloning by somatic cell nuclear transfer / L.C. Smith and J.G. Yoo -- The human embryo: ethical and legal aspects / B.M. Knoppers, S. Bordet and R. Isasi |
Summary |
Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In Human Embryogenesis: Methods and Protocols, internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Human Embryogenesis: Methods and Protocols provides a firm foundation for the successful analysis of the embryogenesis process and an easily accessible description of the limitations and advantages of the techniques proposed, certain to aid all those who wish to further unravel the mysteries of human embryogenesis |
Analysis |
embryos |
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embryogenese |
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embryogenesis |
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embryokloneren |
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embryo cloning |
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embryonale ontwikkeling |
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embryonic development |
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foetale groei |
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fetal growth |
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laboratoriummethoden |
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laboratory methods |
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protocollen |
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protocols |
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Cellular Biology |
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Celbiologie |
Bibliography |
Includes bibliographical references and index |
Notes |
English |
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Print version record |
Subject |
Embryology, Human -- Laboratory manuals
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Fertilization in vitro -- Laboratory manuals
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Cloning -- Laboratory manuals
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Embryology, Experimental -- Moral and ethical aspects -- Laboratory manuals
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Embryology.
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Genetics -- Technique.
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Fertilization in vitro, Human.
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Fertilization in vitro.
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Methodology.
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Human reproductive technology.
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Biology.
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Morphogenesis.
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Reproductive technology.
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Life sciences.
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Physical sciences.
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Therapeutics.
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Genetics
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Embryonic Development
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Genetic Techniques
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Fertilization in Vitro
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Laboratory Manuals
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Methods
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Reproductive Techniques, Assisted
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Embryonic and Fetal Development
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Investigative Techniques
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Biology
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Publication Formats
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Morphogenesis
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Reproductive Techniques
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
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Reproduction
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Biological Science Disciplines
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Publication Characteristics
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Natural Science Disciplines
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Reproductive Physiological Phenomena
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Growth and Development
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Therapeutics
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Disciplines and Occupations
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Physiological Phenomena
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Reproductive and Urinary Physiological Phenomena
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Phenomena and Processes
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methodology.
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biology.
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biological sciences.
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physical sciences.
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treating (health care function)
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Therapeutics
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Reproductive technology
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Physical sciences
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Morphogenesis
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Methodology
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Life sciences
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Human reproductive technology
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Genetics -- Technique
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Fertilization in vitro, Human
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Embryology
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Biology
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Cloning
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Embryology, Human
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Fertilization in vitro
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Développement embryonnaire -- génétique -- Cahiers de laboratoire.
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Techniques génétiques -- Cahiers de laboratoire.
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Embryonalentwicklung
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Labortechnik
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Embryologie humaine -- Manuels de laboratoire.
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Fécondation in vitro -- Manuels de laboratoire.
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Clonage -- Manuels de laboratoire.
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Embryologie expérimentale -- Aspect moral -- Manuels de laboratoire.
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Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Lafond, Julie
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Vaillancourt, Cathy
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ISBN |
9781603270090 |
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1603270094 |
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