| Description |
xx, 261 pages : illustrations ; 24 cm |
| Contents |
Machine derived contents note: Table of contents for Biotechnology : a laboratory course / Jeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo. -- Bibliographic record and links to related information available from the Library of Congress catalog -- Information from electronic data provided by the publisher. May be incomplete or contain other coding. -- Suggested Schedule for Laboratory Exercises. -- Introductory Notes. -- Record Keeping and Safety Rules. -- Format of Student Laboratory Records. -- The Ten Commandments of Record Keeping. -- Safety Rules in the Laboratory. -- Exercise 1: Aseptic Culture and Establishing Pure Cultures. -- Exercise 2: Preparation of Culture Media. -- Exercise 3: The Growth Curve. -- Exercise 4: Isolation of Plasmid DNA from Escherichia coli : The Mini-Prep. -- Exercise 5: Purification, Concentration, and Quantitation of DNA. -- Exercise 6: Large-Scale Isolation of Plasmid DNA by Column Chromatography. -- Exercise 7: Amplification of a lacZ . -- Gene Fragment by the Polymerase Chain Reaction. -- Exercise 8: Restriction Digestion and Agarose Gel Electrophoresis. -- Exercise 9: Southern Transfer. -- Exercise 10: Preparation, Purification, and Hybridization of Probe. -- Exercise 11: Transformation of Saccharomyces cerevisiae . -- Exercise 12: Isolation of Plasmid from Yeast and Escherichia coli Transformation. -- Exercise 13: Protein Assays. -- Exercise 14: Qualitative Assay for Galactosidase in Yeast Colonies. -- Exercise 15: Determination of Galactosidase in Permeabilized Yeast Cells. -- Exercise 16: Assay of Galactosidase in Cell Extracts. -- Exercise 17: Galactosidase Purification. -- Exercise 18: Western Blot: Probe of Protein Blot with Antibody to Galactosidase. -- Appendix 1: Alternate Protocols and Experiments: -- Exercise 1A: Isolation and Characterization of Auxotrophic Yeast Mutants. -- Exercise 2A: Measurement of pH. -- Exercise 3A: Use of the Spectrophotometer. -- Exercise 6A: Isolation of Plasmid DNA: The Maxi-Prep. -- Exercise 10A: Colony Hybridization. -- Appendix 2: Buffer Solutions. -- Appendix 3: Preparation of Buffers and Solutions. -- Appendix 4: Properties of Some Common Concentrated Acids and Bases. -- Appendix 5: Use of Micropipettors. -- Appendix 6: Safe Handling of Microorganisms. Appendix 7: List of Cultures. -- Appendix 8: Storage of Cultures and DNA. -- Appendix 9: Sterilization Methods. -- Appendix 10: Preparation of Stock Solutions for Culture Media. -- Appendix 11: Growth in Liquid Medium. -- Appendix 12: Determination of Viable Cells. -- Appendix 13: Determination of Cell Mass. -- Appendix 14: Determination of Cell Number. -- Appendix 15: Nomenclature of Strains. -- Appendix 16: Glassware and Plasticware. -- Appendix 17: Preparation of Tris and EDTA. -- Appendix 18: Basic Rules for Handling Enzymes. -- Appendix 19: Effects of Common Contaminants on Protein Assays. -- Appendix 20: Manufacturers' and Distributors' Addresses. -- Appendix 21: Surfing the Bionet: World Wide Web Addresses. -- Glossary. -- Subject Index. -- Library of Congress subject headings for this publication: Biotechnology Laboratory manuals |
| Analysis |
Biotechnology |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Biotechnology -- Laboratory manuals.
|
|
Biotechnology.
|
| Author |
Caldwell, Guy A.
|
|
Zachgo, Eve Ann.
|
| LC no. |
95041427 |
| ISBN |
0120845628 (alk. paper) |
|
