| Description |
1 online resource (151 p.) |
| Series |
Pocket Guides to Biomedical Sciences Ser |
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Pocket Guides to Biomedical Sciences Ser
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| Contents |
Cover -- Half Title -- Series -- Title -- Copyright -- Dedication -- Contents -- Preface -- Acknowledgments -- Authors -- Abbreviations -- 1 Genome Editing -- History -- Principle -- Applications of Genome Editing -- Concerns with Genome Editing -- 2 Zinc Finger Nucleases -- History of Zinc Finger Nucleases -- Target Cells (Prokaryotic Cells and Eukaryotic Cells) -- Efficiency -- Specificity -- Monitoring Editing Specificity -- Error Rate -- Versions of ZFNs -- Construct -- Procedure -- ZFN-Mediated Genome Editing -- Applications of ZFNs -- Gene Disruption |
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Gene Disruption in Model Organisms -- Gene Disruption in Mammalian Somatic Cells -- More Complex Types of Disruption -- Homology-Based Genome Editing -- Gene Correction (Allele Editing) -- Gene Addition in Human ES and iPS Cells -- Gene Addition in Plants -- Other Practical Applications of ZFNs -- Advances in Therapy Using ZFNs -- Advantages of ZFNs -- Disadvantages of ZFNs -- 3 TALENs -- History -- Principle -- Procedure -- Efficiency -- Applications of TALENs -- Advantages of TALENs -- Disadvantages of TALENs -- 4 MegaTALs -- Introduction and History -- Ease of Use -- Efficiency -- Specificity |
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Versions of Meganucleases -- Construct -- Principle Behind MegaTALs -- Applications of MegaTALs -- Advantages and Disadvantages -- 5 CRISPR -- History -- Versions of CRISPR -- Mechanism of the CRISPR System -- Genome Editing Using CRISPR -- Applications of CRISPR -- Other Applications of CRISPR in Crops and Livestock -- Other Applications of CRISPR in Humans -- Advantages -- Disadvantages -- How to Counter Off-Target Effects -- 6 Comparison of ZFNs, TALENs, CRISPR, and MegaTALs -- Mechanism of Action -- Nucleases -- DNA Target Recognition -- Recognition Site -- Specificity and Simplicity |
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Targeting Constraints -- Ease of Engineering -- Immunogenicity -- Ease of Ex Vivo Delivery -- Ease of In Vivo Delivery -- Cost Effectiveness -- Speed -- Efficiency of Gene Editing -- Biology at the Breakpoint -- 7 Gene Drives -- Introduction -- History of and Principle behind Gene Drives -- Classifications of Gene Drives -- Standard Drive Systems -- Local Drive Systems -- Daisy Drive Systems -- Threshold Drive Systems -- Combination Drive Systems -- Natural and Synthetic Gene Drives -- Suppression Drives -- Modification Drives -- Threshold Dependent Drive -- Threshold-Independent Drive Systems |
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Temporarily Self-Limiting Drives -- Selfish Genetic Elements -- Over-Replicators -- HEGs (Homing Endonuclease Genes) -- Segregation and Transmission Distorters -- Sex-Ratio Distorters -- Toxin-Antidote Based Drives -- Maternal-Effect Dominant Embryonic Arrest (MEDEA) -- Genetic Underdominance -- Transposable Elements -- Semele System -- Merea System -- Medusa System -- T-Complex or T-Haplotype -- Translocations -- Application of Gene Drives -- Advantages of Gene Drives -- Disadvantages of Gene Drives -- Concerns with Gene Drives -- Regulatory Systems -- 8 Supernumerary B Chromosomes |
| Summary |
Genome-editing methods are becoming routine tools for molecular and cell biologists. Such tools include ZFNs, CRISPR, megaTALs and TALENs. These tools are revolutionizing the creation of precisely manipulated genomes to modify the characteristics of organisms or cells. Additionally, gene drives have altered the way we understand inheritance laws. They give us the ability to have total control of the inheritance of traits of choice and importance. This succinct volume summarizes the history, principles and applications - as well as the advantages and disadvantages - of each of these tools and various kinds of gene drives. The book is part of a program to produce books helpful to students and faculties of science at colleges and universities. This volume in the Pocket Guides to Biomedical Sciences series will help demystify these technologies. The book fills the gap between established conventional methods and the novel and exciting newly introduced tools of genome editing and gene drives. It will help young scientists understand the emerging genome-editing tools and gene drives, thereby promoting related research and adoption. Key Features Extensively reviews the current genome-editing tools and gene drives Clarifies the targeting mechanisms and specificity of genome-editing tools Details many different types of natural and synthetic gene drives Highlights concerns with gene drives and genome-editing tools Related Titles Brown, T. A. Genomes 4 (ISBN 978-0-8153-4508-4) Samuelsson, T. The Human Genome in Health and Disease: A Story in Four Letters (ISBN 978-0-8153-4591-6) Soh, J., et al. Genome Annotation (ISBN 978-1-4398-4117-4) |
| Notes |
Description based upon print version of record |
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Introduction |
| Subject |
Gene editing -- Methodology
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Genetics -- Technique.
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Genetic Techniques
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Genetics -- Technique
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| Form |
Electronic book
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| Author |
Chekera, Ringisai
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Ncube, Clophas Zibusiso
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Shoko, Irvonnie
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Ncube, Berlinda
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Moyo, Thandanani
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Chimbo, Jeffrey Godfrey
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Dube, Jemethious
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Mashiri, Farai Faustos
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Mubani, Moira Amanda
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| ISBN |
9781000463088 |
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1000463087 |
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1000463109 |
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9781000463101 |
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1003165311 |
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9781003165316 |
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