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Book Cover
E-book
Author Iftikhar, Rabia

Title Recent Advances in Plant in Vitro Culture
Published Ashland : Delve Publishing, 2019

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Description 1 online resource (271 pages)
Contents Cover; Half Title Page; Title Page; Copyright Page; About the Author; Table of Contents; List of Figures; List of Tables; List of Abbreviations; Glossary; Preface; Chapter 1 An Introduction to Plant Cell Culture; 1.1. History; Chapter 2 Basic Concepts of Cell Culture; 2.1. Totipotency; 2.2. Cell Morphogenesis; 2.3. Primary Cultures, Confluence, and Passaging of Cells in a Tissue Culture; 2.4. Finite vs. Continuous Cell Lines; 2.5. Suspension and Callus Cultures; 2.6. Cell Culture Medium; 2.7. Cell Culture Conditions; Chapter 3 Setup of a Plant Cell Culture Laboratory
3.1. Basic Design and Maintenance3.2. Equipment Needed in a Plant Cell Culture Lab; 3.3. Care and Maintenance of Laboratory Areas; 3.4. Composition of Nutrient Media; 3.5. Media Selection; Chapter 4 Sterilization Techniques; 4.1. Introduction; 4.2. Work Area; 4.3. Laminar Flow Cabinets; 4.4. Media Sterilization; 4.5. Personnel; 4.6. Explant Sterilization; 4.7. Other Instruments; 4.8. Insect Contamination; 4.9. Methods of Sterilization; 4.10. Types of Surface Disinfectants; 4.11. Avoiding Contaminations; Chapter 5 Micropropagation; 5.1. Introduction; 5.2. Stages of Micropropagation
5.3. Media Preparation5.4. Isolation, Preparation, and Inoculation of The Explant; 5.5. Callus Initiation and Maintenance; 5.6. Sub-Culturing; 5.7. Hardening and Acclimatization; 5.8. Cryopreservation; 5.9. Advantages of Micropropagation; Chapter 6 Types of Plant Tissue Culture Systems; 6.1. Regeneration of Plantlets; 6.2. Regeneration of Seedlings; 6.3. Callus Formation; 6.5. Factors That Affect the Regeneration Capacity of Explants; 6.6. Factors Affecting in Vitro Rooting; 6.7. Methods to Improve Explant Regeneration Capacity; Chapter 7 Embryogenesis; 7.1. Introduction
7.2. Types of EmbryogenesisChapter 8 Synthetic Seeds; 8.1. Introduction; 8.2. Parts of a Typical Synthetic Seed; 8.3. Technique for Production of Synthetic Seeds; 8.4. Selection of Plant Material; 8.5. Encapsulation and Addition of Adjuvants; 8.6. Characteristics of Alginate Matrix; 8.7. The Gelling Process; 8.8. Nutritive Requirements; 8.9. Applications and Limitations; 8.10. Advantages of Synthetic Seed Technology; 8.11. Disadvantages of Synthetic Seed Technology; 8.12. Conclusion; Chapter 9 Organogenesis; 9.1. Introduction; 9.2. Direct or Indirect Organogenesis; 9.3. Medium and Ratios
9.4. Brief History9.5. Terminology; 9.6. Direct Organogenesis; 9.7. Indirect Organogenesis; 9.8. General Account of Organogenesis; 9.9. Protocol for Organogenesis; Chapter 10 Protoplast Culture; 10.1. Introduction; 10.2. Protoplast Isolation, Purification, and Fusion; 10.3. Source of Protoplasts; 10.4. Test for Viability of Protoplast; 10.5. Protoplast Regeneration; 10.6. Somatic Hybridization; 10.7. Cybridization; 10.8. Applications of Somatic Hybridization and Cybridization; Chapter 11 Haploid Plant Culture; 11.1. Introduction; 11.2. Background for Haploid Formation
Notes 11.3. Androgenesis Development of Haploids
Print version record
Subject Plant cell culture.
Plant tissue culture.
Plant tissue culture
Plant cell culture
Form Electronic book
ISBN 1773617699
9781773617695