| Description |
1 online resource (xii, 365 pages) : illustrations (chiefly color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1556 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1556. 1064-3745
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| Contents |
Muscle stem cells : a model system for adult ctem cell biology / DDW Cornelison and Eusebio Perdiguero -- Isolation of muscle stem cells from mouse skeletal muscle / Barbara Gayraud-Morel, Francesca Pala, Hiroshi Sakai, and Shahragim Tajbakhsh -- Primary mouse myoblast purification using magnetic cell separation / Marie Claude Sincennes, Yu Xin Wang, and Michael A. Rudnicki -- Isolation, culture, and immunostaining of skeletal muscle myofibers from WildType and Nestin-GFP mice as a means to analyze satellite cells / Pascal Stuelsatz, Paul Keire, and Zipora Yablonka-Reuveni -- Characterization of drosophila muscle stem cell-like adult muscle precursors / Guillaume Lavergne, Cedric Soler, Monika Zmojdzian, and Krzysztof Jagla -- Using transgenic zebrafish to study muscle stem/progenitor cells / Phong D. Nguyen and Peter D. Currie -- Muscle interstitial cells : a brief field guide to non-satellite cell populations in skeletal muscle / Francesco Saverio Tedesco, Louise A. Moyle, and Eusebio Perdiguero -- Isolation and characterization of vessel-associated stem/progenitor cells from skeletal muscle / Rossana Tonlorenzi, Giuliana Rossi, and Graziella Messina -- Fibro/adipogenic progenitors (FAPs) : Isolation by FACS and culture / Marcela Low, Christine Eisner, and Fabio Rossi -- Single cell gene expression profiling of skeletal muscle-derived cells / Sole Gatto, Pier Lorenzo Puri, and Barbora Malecova -- Engraftment of FACS isolated muscle stem cells into injured skeletal muscle / Matthew Tierney and Alessandra Sacco -- Transplantation of skeletal muscle stem cells / Monica N. Hall, John K. Hall, Adam B. Cadwallader, Bradley T. Pawlikowski, Jason D. Doles, Tiffany L. Elston, and Bradley B. Olwin -- Simultaneous measurement of mitochondrial and glycolytic activity in quiescent muscle stem cells / James G. Ryall -- Monitoring autophagy in muscle stem cells / Laura García-Prat, Pura Muñoz-Cánoves, and Marta Martínez-Vicente -- Mimicking muscle stem cell quiescence in culture : methods for synchronization in reversible arrest / Reety Arora, Mohammed Rumman, Nisha Venugopal, Hardik Gala, and Jyotsna Dhawan -- Methods for observing and quantifying muscle satellite cell motility and invasion in vitro / Dane K. Lund, Patrick McAnulty, Ashley L. Siegel, and DDW Cornelison -- Effects of macrophage conditioned-medium on murine and human muscle cells : analysis of proliferation, differentiation, and fusion / Marielle Saclier, Marine Theret, Rémi Mounier, and Bénédicte Chazaud -- Optimization of stellite cell culture through biomaterials / Sadegh Davoudi and Penney M. Gilbert -- Systematic identification of genes regulating muscle stem cell self-renewal and differentiation / Krishnamoorthy Sreenivasan, Thomas Braun, and Johnny Kim -- Bioinformatics for novel long intergenic noncoding RNA (lincRNA) identification in skeletal muscle cells / Xianlu Peng, Kun Sun, Jiajian Zhou, Hao Sun, and Huating Wang |
| Summary |
This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells. Chapters detail current and updated methods for muscle stem cell isolation, culture, molecular analysis, cellular analysis, and reintroduction in vivo as well as protocols for studying myogenic stem cells in non-mammalian model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Muscle Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF of title page (Springer Protocols, viewed March 3, 2017) |
| Subject |
Stem cells -- Laboratory manuals
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Cell culture -- Laboratory manuals
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Stem cells.
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Cell physiology.
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Muscle, Skeletal -- cytology
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Stem Cells
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Cell Physiological Phenomena
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Cellular biology (cytology)
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Cell physiology
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Cell culture
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Stem cells
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Perdiguero, Eusebio, 1968- editor
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Cornelison, D. D. W., 1967- editor.
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| ISBN |
9781493967711 |
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1493967711 |
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