| Description |
1 online resource (xxi, 280 pages) : illustrations |
| Contents |
Transposon Mutagenesis of Escherichia coli. -- Recombinant DNA and Cloning. -- Southern Blot Analysis. -- Plant Genomic Southern Blotting with Probes for Low- and High-Copy-Number Genes. -- RNA Purification and Northern Blot Analysis. -- Polymerase Chain Reaction. -- Appendices. -- Glossary. -- Subject Index |
| Summary |
This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Key Features * Includes a glossary of over 200 terms important for understanding molecular biology * Uses an inexpensive source of eukaryotic cells - great for schools on a budget * Includes Methods Locator that provides instant access to the latest methods * Contain clearly written, easy-to-follow, student-tested instructions: * Sterile techniques * Phage titration * Gel electrophoresis of DNA * Restriction enzyme digestion * Plasmid isolation * Transformation of E. Coli * Recombinant DNA cloning * Nick translation labeling * Nonradioactive primer labelling * Nonradioactive DNA detection * Southern blotting * Colony hybridization * Purification of plant DNA * RNA purification * Northern blotting * Purification of poly A+ RNA * Polymerase chain reaction (PCR) |
| Analysis |
Molecular biology |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Molecular biology -- Laboratory manuals
|
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Molecular biology -- Experiments
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Molecular Biology
|
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SCIENCE -- Life Sciences -- Molecular Biology.
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Molecular biology
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Molecular biology -- Experiments
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Genetik
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Methode
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Molekularbiologie
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Molekulargenetik
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Biologie moléculaire -- Manuels de laboratoire.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
|
| ISBN |
9780123977205 |
|
0123977207 |
|
9780080536835 |
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0080536832 |
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