Title Methods in biological oxidative stress / edited by Kenneth Hensley, Robert A. Floyd

Published Totowa, N.J. : Humana Press, ©2003

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Description 1 online resource (xv, 215 pages) : illustrations Series Methods in pharmacology and toxicology Methods in pharmacology and toxicology. Contents Methods in Biological Oxidative Stress -- Foreword -- Preface -- Contents -- Contributors -- Lipids -- 1 Measurement of Fat-Soluble Vitamins and Antioxidants by HPLC With Electrochemical Array Detection -- 2 Analysis of Aldehydic Markers of Lipid Peroxidation in Biological Tissues by HPLC with Fluorescence Detection -- 3 Measurement of Isofurans by Gas Chromatography�Mass Spectrometry/ Negative Ion Chemical Ionization -- 4 Analysis of F2-Isoprostanes by Gas Chromatography�Mass Spectrometry/ Negative Ion Chemical Ionization 5 Measurement of F4-Neuroprostanes by Gas Chromatography�Mass Spectrometry/ Negative Ion Chemical Ionization6 Immunoassays for Lipid Peroxidation End Products: One-Hour ELISA for Protein-Bound Acrolein and HNE -- 7 Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biological Matrices -- 8 HPLC With Electrochemical and Photodiode Array Detection Analysis of Tocopherol Oxidation and Nitration Products in Human Plasma -- DNA, Protein, and Amino Acids 9 Electron Paramagnetic Resonance Spin-Labeling Analysis of Synaptosomal Membrane Protein Oxidation10 Gas Chromatography�Mass Spectrometric Analysis of Free 3-Chlorotyrosine, 3-Bromotyrosine, Ortho-Tyrosine, and 3-Nitrotyrosine in Biological Fluids -- 11 Isotope Dilution Gas Chromatography� Mass Spectrometric Analysis of Tyrosine Oxidation Products in Proteins and Tissues -- 12 Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring and Ridit Analysis -- 13 Detection of Aldehydic DNA Lesions Using Aldehyde Reactive Probe 14 Analysis of Neuroketal Protein Adducts by Liquid Chromatography�Electrospray Ionization/Tandem Mass Spectrometry15 Measurement of Isoketal Protein Adducts by Liquid Chromatography�Electrospray Ionization/Tandem Mass Spectrometry -- 16 Bioassay of 2'-Deoxyguanosine/8-Hydroxy-2'- Deoxyguanosine by HPLC With Electrochemical/ Photodiode Array Detection -- 17 HPLC With Electrochemical Detection Analysis of 3-Nitrotyrosine in Human Plasma -- Reactive Oxygen Species and Reactive Nitrogen Species -- 18 Protein Carbonyl Levels� An Assessment of Protein Oxidation 19 Fluorogenic Analysis of H2O2 in Biological Materials20 Detection of Reactive Oxygen Species by Flow Cytometry -- 21 Nitrite Determination by Colorimetric and Fluorometric Griess Diazotization Assays: Simple, Reliable, High-Throughput Indices of Reactive Nitrogen Species in Cell-Culture Systems -- 22 Protein Carbonyl Determination Using Biotin Hydrazide -- 23 Real-Time, In-Vivo Measurement of Nitric Oxide Using Electron Paramagnetic Resonance Spectroscopic Analysis of Biliary Flow -- Index Summary Free radicals and reactive oxidizing agents are now recognized as causes or contributing factors in inflammation and other disease states and have become a major focus of interest in pharmacology, toxicology, physiology, and biochemistry. In Methods in Biological Oxidative Stress, expert researchers detail their best experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established standard protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, and to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods for measuring lipid oxidation products. Each technique is described in step-by-step detail to ensure robust results, with many presented by the experimenters who have led their development. Useful notes explain the pitfalls to avoid and the chemical principles underlying each method. Authoritative and highly practical, Methods in Biological Oxidative Stress provides laboratory researchers and technicians a unique collection of standard, easy-to-use experimental methods for oxidative stress bioanalysis, plus cutting-edge instrumental techniques using HPLC, mass spectrometry, and EPR spectrometry Bibliography Includes bibliographical references and index Notes English Print version record Subject Oxidative stress -- Laboratory manuals Free Radicals -- physiology Oxidative Stress -- physiology Antioxidants -- physiology MEDICAL -- Pathology. MEDICAL -- Pathophysiology. Oxidative stress Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Hensley, Kenneth Floyd, Robert A., 1940- ISBN 0896038157 9780896038158 1592594247 9781592594245 1280842962 9781280842962 9786610842964 6610842965

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