| Description |
1 online resource (xv, 463 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1879 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1879. 1064-3745
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| Contents |
In vivo genetic alteration and lineage tracing of single stem cells by live imaging / Olivia Farrelly, Paola Kuri, and Panteleimon Rompolas -- Ex vivo imaging and genetic manipulation of mouse hair follicle bulge stem cells / Daniel Haensel, Melissa A. McNeil, and Xing Dai -- Quantitative detection of low-abundance transcripts at single-cell level in human epidermal keratinocytes by digital droplet reverse transcription-polymerase chain reaction / Frédéric Auvré, Julien Coutier, Michèle T. Martin, and Nicolas O. Fortunel -- Qualitatively monitoring binding and expression of the transcription factors Sp1 and NFI as a useful tool to evaluate the quality of primary cultured epithelial stem cells in tissue reconstruction / Gae¨tan Le-Bel, Sergio Cortez Ghio, Danielle Larouche, Lucie Germain, and Sylvain L. Guérin -- Ribonucleoproteins mediated efficient in vivo gene editing in skin stem cells / Wenbo Wu and Ting Chen -- Isolation and characterization of cutaneous epithelial stem cells / Stephanie R. Gillespieand David M. Owens -- Interfollicular epidermal stem cells : boosting and rescuing from adult skin / Mariana T. Cerqueira, Ana M. Frias, Rui L. Reis, and Alexandra P. Marques -- Whole-mount immunofluorescent staining coupled to multicolor lineage tracing model for analyzing the spatiotemporal organization of epidermal stem cells / Edwige Roy and Kiarash Khosrotehrani -- Isolation and enrichment of newborn and adult skin stem cells of the interfollicular epidermis / Stefano Sol, Dario Antonini, and Caterina Missero -- Isolation and cultivation of epidermal (stem) cells / Xusheng Wang, Shiyang Dong, and Yaojiong Wu -- One-step simple isolation method to obtain both epidermal and dermal stem cells from human skin specimen / Hua Qian, Xue Leng, Jie Wen, Qian Zhou, Xin Xu, and Xunwei Wu -- Isolation and cultivation of skin-derived precursors / Xiaoxiao Wang, Shiyang Dong, and Yaojiong Wu -- Magnetic-based cell isolation technique for the selection of stem cells / Petek Korkusuz, Sevil Ko¨se, Nilgu¨n Yersal, and Selin O ¨nen -- Isolation of human skin epidermal stem cells based on the expression of endothelial protein C receptor / Meilang Xue, Suat Dervish, and Christopher J. Jackson -- Decellularized bSIS-ECM as a regenerative biomaterial for skin wound repair / Mahmut Parmaksiz, Ays¸e Eser Elc¸in, and Yas¸ar Murat Elc¸in -- Protocols for full thickness skin wound repair using prevascularized human mesenchymal stem cell sheet / Lei Chen, Daniel Radke, Shaohai Qi, and Feng Zhao -- Cultivation of adipose-derived stromal cells on intact amniotic membrane-based scaffold for skin tissue engineering / Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, and Amir Bajouri -- Amniotic membrane seeded fetal fibroblasts as skin substitute for wound regeneration / Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, and Amir Bajouri -- Skin wound healing : refractory wounds and novel solutions / Gabriel M. Virador, Lola de Marcos, and Victoria M. Virador -- Isolation, culture, and motility measurements of epidermal melanocytes from GFP-expessing reporter mice / Lina Dagnino and Melissa Crawford -- Melanoblasts as multipotent cells in murine skin / Tsutomu Motohashi and Takahiro Kunisada -- Regeneration of mouse skin melanocyte stem cells in vivo and in vitro / Ke Yang, Weiming Qiu, Pei-Rong Gu, and Mingxing Lei -- Interactions between epidermal keratinocytes, dendritic epidermal T-cells, and hair follicle stem cells / Krithika Badarinath, Abhik Dutta, Akshay Hegde, Neha Pincha, Rupali Gund, and Colin Jamora -- Isolating immune cells from mouse embryonic skin / Ambika S. Kurbet and Srikala Raghavan -- Direct conversion of mouse embryonic fibroblasts into neural crest cells / Tsutomu Motohashi and Takahiro Kunisada -- High-titer production of HIV-based lentiviral vectors in roller bottles for gene and cell therapy / Hazal Banu Olgun, Hale M. Tasyurek, Ahter Dilsad Sanlioglu, and Salih Sanlioglu -- High-grade purification of third-generation HIV-based lentiviral vectors by anion exchange chromatography for experimental gene and stem cell therapy applications / Hazal Banu Olgun, Hale M. Tasyurek, Ahter D. Sanlioglu, and Salih Sanlioglu -- Full-thickness human skin equivalent models of atopic dermatitis / Gopu Sriram, Paul Lorenz Bigliardi, and Mei Bigliardi-Qi -- Human hair follicle associated-pluripotent (hHAP) stem cells differentiate to cardiac muscle cells / Robert M. Hoffman -- Isolation of normal fibroblasts and their cancer-associated counterparts (CAFs) for biomedical research / Barbora Dvorˇánková , Luká sˇ Lacina, and Karel Smetana Jr. -- Isolation of cancer stem cells from squamous cell carcinoma / Silvia Fontenete and Mirna Perez-Moreno -- Identification of human cutaneous squamous cell carcinoma cancer stem cells / Carlotta Olivero, Huw Morgan, and Girish K. Patel -- Identification of human cutaneous basal cell carcinoma cancer stem cells / Huw Morgan, Carlotta Olivero, and Girish K. Patel |
| Summary |
This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed January 29, 2019) |
| Subject |
Stem cells.
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Skin -- Cytology
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Stem Cells
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Skin -- cytology
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Cytological Techniques -- methods
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Epithelial Cells -- cytology
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Cellular biology (cytology)
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Skin -- Cytology
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Stem cells
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| Genre/Form |
Electronic books
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Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Turksen, Kursad, editor.
|
| ISBN |
9781493988709 |
|
1493988700 |
|
1493988697 |
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9781493988693 |
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