| Description |
1 online resource : text file, PDF |
| Series |
Clinical and Biochemical Analysis |
| Contents |
Cover; Half Title; Title Page; Copyright Page; Preface; Contents; Contributors; 1 Theoretical and Technical Considerations for the Use of Monoclonal Antibodies in Diagnostic Immunohistochemistry; PART I; THEORETICAL CONSIDERATIONS; Monoclonal Antibodies: The Concept; Molecular Variability of Immunoglobulins; Generation of Variability; Generation of Variability: Practical Issues; Why Bother with MoAb?; Consistency; Specificity; Nature of Cross-Reactivity; Problems Associated with Specificity; How to Make MoAb; Spontaneous Generation of MoA b; Hybridoma Technology; PART II |
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TECHNICAL CONSIDERATIONSSpecimen Procurement and Processing; Specimen Size; Use of Frozen Sections; Touch-Imprint Preparations and Cytological Specimens; Fixed Specimens; Effects of Decalcifying Solutions on Immunoreactivity; Freeze-Dried Tissues; Microwave-Fixed Specimens; Clearing Agents for Paraffin-Embedded Tissues; Blocking Steps; Incubation with Primary Antibodies; Immunohistochemical Development Systems; Direct, Labeled Antibody Methods; Indirect, Unlabeled Antibody Methods; Haptenated Antibody-Antihapten (HAAH) Procedures; Antibody-Staphylococcal Protein A-PAP (ASPAPAP) Procedures |
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Colloidal Metal-Antibody Complex ProceduresCombined Antibody-Enzyme Procedures; Chromogens; Postchromogenic Enhancement Methods; Counterstains; Troubleshooting; Lack of Staining in Both Positive Control Sections and Test Tissues Possibilities.; Positive Controls Stain Appropriately, but Test Tissue Does Not Possibilities.; Background Staining Is Unacceptably High Possibilities.; Need for Negative Controls; Workup of Newly Accessioned Antibodies; REFERENCES; 2 Monoclonal Antibodies to Cytokeratins in Diagnostic Immunocytochemistry; BIOLOGY OF CYTOKERATINS |
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DIAGNOSTIC IMMUNOCYTOCHEMISTRY OF CYTOKERATINSDIFFERENTIAL DIAGNOSIS OF EPITHELIAL NEOPLASMS USING ANTICYTOKERATIN MONOCLONAL ANTIBODIES; COMPARISON OF CYTOKERATIN ANTIBODIES; CYTOKERATIN EXPRESSION IN NONEPITHELIAL NEOPLASMS: GERM CELL NEOPLASMS, SARCOMAS, AND NEUROECTODERMAL TUMORS; COEXPRESSION OF CYTOKERATIN WITH OTHER INTERMEDIATE FILAMENT PROTEINS; FIXATION, IMMUNOCYTOCHEMICAL TECHNIQUE, AND INTERPRETATION OF IMMUNOSTAINS; CONCLUSIONS; REFERENCES; 3 Monoclonal Antibodies to Vimentin; BIOCHEMICAL CHARACTERISTICS OF VIMENTIN AND RELATIONSHIP TO OTHER INTERMEDIATE FILAMENT PROTEINS |
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SENSITIVITY AND SPECIFICITY DATATECHNICAL CONSIDERATIONS; PRACTICAL APPLICATIONS OF VIMENTIN ANTIBODIES IN THE DIAGNOSIS OF NEOPLASTIC DISEASE; Spindle Cell Neoplasms (Table 2); Tumors of Skin; Tumors of Soft Tissue; Other Neoplasms That May Exhibit a Prominent Spindle Cell Component; Undifferentiated Small Round Cell Tumors; Epithelioid and Pleomorphic Tumors; Regional Differential Diagnosis of Carcinomas; Primary Intracranial Tumors; PRACTICAL APPLICATIONS OF VIMENTIN ANTIBODIES IN NONNEOPLASTIC DISEASES; PITFALLS IN THE INTERPRETATION OF IMMUNOSTAINS TO VIMENTIN; REFERENCES |
| Subject |
Allergy.
|
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Clinical immunology.
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Allergy
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Clinical immunology
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| Form |
Electronic book
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| Author |
Wick, Mark, editor
|
| ISBN |
9781466593497 |
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1466593490 |
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