| Description |
1 online resource (xii, 483 pages) : illustrations (some color) |
| Series |
Methods in Pharmacology and Toxicology, 1940-6053 |
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Methods in pharmacology and toxicology, 1557-2153
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| Contents |
PART I. GENOTOXICITY ASSAYS -- 1. Ames Test (Bacterial Reverse Mutation Test): Why, When, and How to Use / Araceli Pillco and Eduardo de la Peña -- 2. The Ames II and Ames MPF Penta I Assay: A Liquid Microplate Format Modification of the Classic Ames Test / Sini Flückiger-Isler and Markus Kamber -- 3. Revised Procedure of the Bacterial Reverse Mutation Test for Genotoxic Evaluation of Nanoparticles / Christophe Pagnout, Stéphane Jomini, and Pascale Bauda -- 4. The Control of Hydrophobic Compound Exposure in In Vitro Tests for Genotoxicity / Kilian E.C. Smith -- 5. The In Vitro Micronucleus Assay and FISH Analysis / Lucia Migliore, Sebastiano Di Bucchianico, and Chiara Uboldi -- 6. The In Vivo Rodent Micronucleus Test / Edson Luis Maistro -- 7. Chromosomal Aberration Test Utilities In Vitro and In Vivo / Ana Paula A. Guimarães, Adriana C. Guimarães, Diego Á. Alcântara, Luiz Raimundo Cunha, Patrícia L. Lima, Marne C. Vasconcellos, Raquel C. Montenegro, Bruno M. Soares, Marucia M. Amorim, and Rommel R. Burbano -- 8. Chromosome Painting of Mouse Peripheral Blood and Spleen Tissues / Dayton M. Petibone, James D. Tucker, and Suzanne M. Morris -- 9. T-Cell Receptor Mutation Assay for Monitoring Human Genotoxic Exposure / Seishi Kyoizumi -- 10. The Human RBC PIG-A Gene Mutation Assay / Vasily N. Dobrovolsky and Robert H. Heflich -- 11. The Applicable Use of the HPRT Gene Mutation Assay as a Practical Tool in Mutagenesis and DNA Repair Studies / Zoulikha M. Zaïr and George E. Johnson -- 12. The Comet Assay: High Throughput Use of FPG / Amaya Azqueta and Andrew R. Collins -- 13. The Comet Assay In Vivo in Humans / Carla Costa and João Paulo Teixeira -- 14. Analysis of Nanoparticle-Induced DNA Damage by the Comet Assay / Julia Catalán, Satu Suhonen, Anna Huk, and Maria Dusinska -- 15. The Comet Assay in Drosophila: Neuroblast and Hemocyte Cells / L. María Sierra, Erico R. Carmona, Leticia Aguado, and Ricard Marcos -- 16. The SMART Assays of Drosophila: Wings and Eyes as Target Tissues / Ricard Marcos, L. María Sierra, and Isabel Gaivão -- 17. Testing the Genotoxic Potential of Nanomaterials Using Drosophila / Mohamed A. Abdalaziz, Balasubramanyam Annangi, and Ricard Marcos -- 18. Transgenic Rodent Gene Mutation Assay in Somatic Tissues / John D. Gingerich, Lynda Soper, Christine L. Lemieux, Francesco Marchetti, and George R. Douglas -- 19. The Mouse Lymphoma Assay / Tao Chen, Xiaoqing Guo, and Martha M. Moore -- 20. Bhas 42 Cell Transformation Assay for Genotoxic and Non- Genotoxic Carcinogens / Kiyoshi Sasaki, Anna Huk, Naouale El Yamani, Noriho Tanaka, and Maria Dusinska |
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PART II. DNA REPAIR ASSAYS -- 21. Methods for Measuring DNA Repair: Introduction and Cellular Repair / Andrew R. Collins and Amaya Azqueta -- 22. A Standardized Protocol for the In Vitro Comet-Based DNA Repair Assay / Jana Slyskova, Sabine A.S. Langie, Isabel Gaivão, Andrew R. Collins, and Amaya Azqueta -- 23. Use of the Comet Assay to Study DNA Repair in Drosophila melanogaster / Isabel Gaivão, Rubén Rodríguez, and L. María Sierra -- 24. Use of RNA Interference to Study DNA Repair / Elise Fouquerel, Jianfeng Li, Andrea Braganza, Zhongxun Yu, Ashley R. Brown, Xiao-Hong Wang, Sandy Schamus, David Svilar, Qingming Fang, and Robert W. Sobol |
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PART III. OTHERS -- 25. The LacZ Plasmid-Based Transgenic Mouse Model: An Integrative Approach to Study the Genotoxicity of Nanomaterials / Henriqueta Louro, Miguel Pinto, Nádia Vital, Ana M. Tavares, Pedro M. Costa, and Maria João Silva |
| Summary |
Genotoxicity and DNA Repair: A Practical Approach provides a key reference for determining how to analyze the genotoxic activity of molecules or materials and, at the same time, serves as a useful tool for researchers in the Environmental Mutagenesis and DNA Repair fields. Focused on genotoxicity assays recommended by the "OECD guidelines for the testing of chemicals", this volume also covers other useful assays, such as some gene mutation assays, the comet assay in different species and applications, and the SMART assays of Drosophila. For all the assays, the book presents brief theoretical introductions to the topics and updated standard and modified step-by-step protocols to perform them. Special emphasis is placed on the analysis of nanoparticles, including an integrative approach analysis. The DNA Repair section includes several assays that provide information on repair activity in vitro and in vivo, as well as recent applications to study DNA repair in humans, cell cultures, and animal models. As a volume in the Methods in Pharmacology and Toxicology series, the chapters contain the kind of detail and key implementation advice that ensures reproducible results in the lab |
| Analysis |
farmacologie |
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pharmacology |
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toxicologie |
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toxicology |
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biomedische wetenschappen |
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biomedicine |
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humane genetica |
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human genetics |
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Medicine (General) |
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Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed Sept. 4, 2014) |
| Subject |
Genetic toxicology -- Laboratory manuals
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DNA repair -- Laboratory manuals
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DNA repair.
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Mutagenicity Tests -- methods
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DNA Repair
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Genetic toxicology
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DNA repair
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Biology -- Laboratory manuals
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Sierra, L. María, editor
|
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Gaivão, Isabel, editor
|
| ISBN |
9781493910687 |
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149391068X |
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1493910671 |
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9781493910670 |
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