| Description |
1 online resource (xxxiv, 640 pages) : illustrations |
| Series |
Methods in enzymology ; v. 197 |
|
Methods in enzymology ; v. 197
|
| Contents |
Assay strategies and methods for phospholipases / Laure J. Reynolds [and others] -- Utilization of labeled Escherichia coli as phospholipase substrate / Peter Elsbach, Jerrold Weiss and A. Dennis Edward -- Nuclear magnetic resonance spectroscopy to follow phospholipase kinetics and products / Mary F. Roberts and A. Dennis Edward -- Monolayer techniques for studying phospholipase kinetics / S. Ransac [and others] -- Thio-based phospholipase assay / Lin Yu, Edward A. Dennis and A. Dennis Edward -- Chromogenic substrates and assay of phospholipases A2 / Wonhwa Cho, Ferenc J. Kézdy and A. Dennis Edward -- Coupled enzyme assays for phospholipase activities with plasmalogen substrates / M.S. Jurkowitz-Alexander [and others] -- Phospholipase A2 assays with fluorophore-labeled lipid substrates / H. Stewart Hendrickson and A. Dennis Edward -- Assays of phospholipases on short-chain phospholipids / Mary F. Roberts and A. Dennis Edward -- Phospholipase A2-catalyzed hydrolysis of vesicles: Uses of interfacial catalysis in the scooting mode / Mahendra Kumar Jain, Michael H. Gelb and A. Dennis Edward -- Assay of phospholipases C and D in presence of other lipid hydrolases / Karl Y. Hostetler [and others] -- Preparation of alkyl ether and vinyl ether substrates for phospholipases / Fritz Paltauf, Albin Hermetter and A. Dennis Edward -- Inositol phospholipids and phosphates for investigation of intact cell phospholipase C substrates and products / Michael R. Hanley [and others] -- Chromatographic analysis of phospholipase reaction products / Merle L. Blank, Fred Snyder and A. Dennis Edward -- Assays for measuring arachidonic acid release from phospholipids / Floyd H. Chilton and A. Dennis Edward -- Phosphoinositide-specific phospholipase C activation in brain cortical membranes / Michael A. Wallace [and others] -- Quantitative analysis of water-soluble products of cell-associated phospholipase C- and phospholipase D-catalyzed hydrolysis of phosphatidylcholine / Donald A. Kennerly and A. Dennis Edward -- Dissection and sequence analysis of phospholipases A2 / Robert L. Heinrikson and A. Dennis Edward -- Cloning, expression, and purification of porcine pancreatic phospholipase A2 and mutants / H.M. Verheij, G.H. de Haas and A. Dennis Edward -- Preparation of antibodies to phospholipases A2 / Makoto Murakami [and others] |
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Thermodynamics of phospholipase A2-ligand interactions / Rodney L. Biltonen [and others] -- Activation of phospholipase A2 on lipid bilayers / John D. Bell, Rodney L. Biltonen and A. Dennis Edward -- Phospholipase stereospecificity at phosphorus / Karol S. Bruzik, Ming-Daw Tsai and A. Dennis Edward -- Phospholipase A2: Microinjection and cell localization techniques / Dafna Bar-Sagi and A. Dennis Edward -- Analysis of lipases by radiation inactivation / E.S. Kempner [and others] -- Phosphorylation of phospholipase C in vivo and in vitro / Jill Meisenhelder, Tony Hunter and A. Dennis Edward -- Detergent-resistant phospholipase A1 from Escherichia coli membranes / Yasuhito Nakagawa [and others] -- Phospholipase A1 activity of guinea pig pancreatic lipase / Ama Gassama-Diagne [and others] -- Purification of rat kidney lysosomal phospholipase A1 / Karl Y. Hostetler, Michael F. Gardner and A. Dennis Edward -- Purification and substrate specificity of rat hepatic lipase / Moseley Waite [and others] -- Human postheparin plasma lipoprotein lipase and hepatic triglyceride lipase / Richard L. Jackson, Larry R. McLean and A. Dennis Edward -- Phospholipase activity of milk lipoprotein lipase / Gunilla Bengtsson-Olivecrona, Thomas Olivecrona and A. Dennis Edward -- Cobra venom phospholipase A2: Naja naja naja / Laure J. Reynolds, Edward A. Dennis and A. Dennis Edward -- Phospholipase A2 from rat liver mitochondria / H. van den Bosch [and others] -- Assay and purification of phospholipase A2 from human synovial fluid in rheumatoid arthritis / Ruth M. Kramer, R. Blake Pepinsky and A. Dennis Edward -- Purification of mammalian nonpancreatic extracellular phospholipases A2 / Shuntaro Hara [and others] -- Spleen phospholipases A2 / Hiromasa Tojo [and others] -- Purification and characterization of cytosolic phospholipase A2 activities from canine myocardium and sheep platelets / Stanley L. Hazen [and others] -- Platelet-activating factor acetylhydrolase in human erythrocytes / Diana M. Stafforini [and others] -- Phospholipase activity of lecithin-cholesterol acyltransferase / Christopher J. Fielding, Xavier Collet and A. Dennis Edward -- Lysophospholipases from Escherichia coli / Ken Karasawa, Shoshichi Nojima and A. Dennis Edward -- Phospholipase B from Penicillium notatum / Kunihiko Saito [and others] -- Lysophospholipases I and II from P388D1 macrophage-like cell line / Ying Yi Zhang [and others] |
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Lysophospholipases from bovine liver / H. van den Bosch [and others] -- Purification of lysophospholipase and lysophospholipase-transacylase from rabbit myocardium / Richard W. Gross and A. Dennis Edward -- Lysoplasmalogenase: solubilization and partial purification from liver microsomes / M.S. Jurkowitz-Alexander, L.A. Horrocks and A. Dennis Edward -- Phosphatidylinositol-specific phospholipases C from Bacillus cereus and Bacillus thuringiensis / O. Hayes Griffith [and others] -- Assays of phosphoinositide-specific phospholipase C and purification of isozymes from bovine brains / Sue Goo Rhee [and others] -- Properties of phospholipase C isozymes / Tadaomi Takenawa [and others] -- Phosphatidylinositol-specific phospholipase C from human platelets / Yoshinori Nozawa, Yoshiko Banno and A. Dennis Edward -- Purification of guinea pig uterus phosphoinositide-specific phospholipase C / C. Frank Bennett [and others] -- Human acid sphingomyelinase from human urine / L.E. Quintern, K. Sandhoff and A. Dennis Edward -- Purification of neutral sphingomyelinase from human urine / Subroto Chatterjee, Nupur Ghosh and A. Dennis Edward -- Phosphatidate phosphatase from yeast / George M. Carman, Yi-Ping Lin and A. Dennis Edward -- Characterization and assay of phosphatidate phosphatase / Ashley Martin [and others] -- Glycosylphosphatidylinositol-specific phospholipase D / Kuo-Sen Huang [and others] -- Solubilization and purification of rat tissue phospholipase D / Mutsuhiro Kobayashi, Julian N. Kanfer and A. Dennis Edward -- Lysophospholipase D / Robert L. Wykle, Jay C. Strum and A. Dennis Edward |
| Summary |
This volume on Phospholipases, the first of its kind, presents techniques for the assay, purification, and characterization of these enzymes, which are not only degradative enzymes of lipid metabolism but are also key enzymes in the signal transduction pathway |
| Analysis |
Phospholipases |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Print version record |
| Subject |
Phospholipases.
|
|
Phospholipases -- analysis.
|
| Form |
Electronic book
|
| Author |
Dennis, Edward A.
|
| ISBN |
012182098X (electronic bk.) |
|
9780121820985 (electronic bk.) |
|
