| Description |
1 online resource (xviii, 565 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1922 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1922. 1064-3745
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| Contents |
Microdissection and isolation of mouse dental epithelial cells of continuously growing mouse incisors / Hidemitsu Harada and Keishi Otsu -- Establishment of an immortalized mouse bmp2 knockout dental papilla mesenchymal cell line / Wen'an Xu and Shuo Chen -- Establishment of stable cell lines from primary human dental pulp stem cells / Elizabeth Guirado, Youbin Zhang, and Anne George -- Isolation of dental stem cell-enriched populations from continuously growing mouse incisors / Anamaria Balic -- Application of cell lineage tracing combined with immunofluorescence in the study of dentinogenesis / Yan Jing, Chaoyuan Li, and Jian Q. Feng -- Tissue recombination and kidney capsule transplantation assays for the study of epithelial-mesenchymal interactions / Lucia Jimenez-Rojo and Thimios A. Mitsiadis |
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Dental mesenchymal stem cells : dental pulp stem cells, periodontal ligament stem cells, apical papilla stem cells, and primary teeth stem cells-isolation, characterization, and expansion for tissue engineering / Mey Al-Habib and George T.-J. Huang -- Phenotypic identification of dental pulp mesenchymal stem/stromal cells subpopulations with multiparametric flow cytometry / Maxime Ducret, Jean-Christophe Farges, Marielle Pasdeloup, Emeline Perrier-Groult, Andreas Mueller, Freþdeþric Mallein-Gerin, and Hugo Fabre -- Dental pulp stem cells: isolation, characterization, expansion, and odontoblast differentiation for tissue engineering / Qing Dong, Yuanyuan Wang, Fatemeh Mohabatpour, Li Zheng, Silvana Papagerakis, Daniel Chen, and Petros Papagerakis -- In vitro analysis of intramolecular signaling events in PDLSCs using confocal and TIRF microscopy / Annette Merkel and Anne George -- Mouse model to study reparative dentinogenesis |
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R.C. Babb, D. Chandrasekaran, L.K. Zaugg, and P.T. Sharpe -- Multiwalled carbon nanotubes for dental applications / Petros Kechagioglou, Eleftherios Andriotis, Petros Papagerakis, and Silvana Papagerakis -- Peptide-mediated biomimetic regrowth of human enamel in situ / Kaushik Mukherjee, Qichao Ruan, and Janet Moradian-Oldak -- Bioengineering tooth bud constructs using GelMA hydrogel / Elizabeth E. Smith and Pamela C. Yelick -- Whole-mount in situ hybridization of mouse embryos using DIG-labeled RNA probes / Jingyi Wu and Xiaofang Wang -- In situ hybridization on mouse paraffin sections using DIG-labeled RNA probes / Jingyi Wu, Jian Q. Feng, and Xiaofang Wang -- Methods for in situ protein visualization in dental mineralized tissues / D. Hotton, A. Berdal, and A. Bolanos -- In situ hybridization in mineralized tissues : the added value of LNA probes for RNA detection / G. Lignon, D. Hotton, A. Berdal, and A. Bolanos |
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Immunofluorescence procedure for developing enamel tissues / Xu Yang and Elia Beniash -- Silver-albumin tissue staining protocol to visualize odontogenesis in whole embryos / Julia C. Boughner and David M.L. Cooper -- Isolation of SIBLING proteins from bone and dentin matrices / Jingyi Wu and Xiaofang Wang -- Immunohistochemical co-localization of amelogenin and ameloblastin in developing enamel matrix / Rucha Arun Bapat and Janet Moradian-Oldak -- Expression and purification of recombinant mouse ameloblastin in E. coli / Jingtan Su, Rucha Arun Bapat, and Janet Moradian-Oldak -- Protocols for Studying formation and mineralization of dental tissues in vivo : extraction protocol for isolating dentin matrix proteins from developing teeth / Yasuo Yamakoshi, Jan C.-C. Hu, Mari M. Saito, and James P. Simmer -- Purification of Developing enamel matrix proteins using preparative SDS-PAGE / Steven J. Brookes and Claire M. Gabe |
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Using imageJ (FIJI) to analyze and present x-ray CT images of enamel / Steven J. Brookes -- Scanning electron microscopy (SEM) methods for dental enamel / Steinar Risnes, Muhammad Saeed, and Amer Sehic -- Microcomputed tomography imaging in odontogenesis studies / Kostas Verdelis and Phil Salmon -- Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) for the examination of dental hard tissues / Victor E. Arana-Chavez and Leticia S. Castro-Filice -- Rodent dental fluorosis model: extraction of enamel organ from rat incisors / M. Suzuki and J.D. Bartlett -- Three-dimensional assessment of crown size and eruption space for developing third molars : data collection techniques based on cone-beam computed tomography (CBCT) / D.F. Marchiori, G.V. Packota, and J.C. Boughner -- Protocols to study dental caries in vitro : microbial caries models / Bennett T. Amaechi, Livia M.A. Tenuta, Antonio P. Ricomini Filho, and Jaime A. Cury |
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In vitro caries models for the assessment of novel restorative materials / Basma Sulaiman Ghandourah, Anna Lefkelidou, Raed Said, Xanthippi Chatzistavrou, Susan Flannagan, Carlos Gonza les-Cabezas, Christopher J. Fenno, Li Zheng, Silvana Papagerakis, and Petros Papagerakis -- Protocols to study dental caries in vitro : pH cycling models / Bennett T. Amaechi -- In vivo rodent models for studying dental caries and pulp disease / June Hsiao, Yuanyuan Wang, Li Zheng, Ruirui Liu, Raed Said, Lubomir Hadjiyski, Heekon Cha, Tatiana Botero, Xanthippi Chatzistavrou, Qing Dong, Silvana Papagerakis, and Petros Papagerakis -- Protocol GenoDENT : implementation of a new NGS panel for molecular diagnosis of genetic disorders with orodental involvement |
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Tristan Rey, Julien Tarabeux, Benedicte Gerard, Marion Delbarre, Antony Le Bechec, Corinne Stoetzel, Megana Prasad, Virginie Laugel-Haushalter, Marzena Kawczynski, Jean Muller, Jamel Chelly, Helene Dollfus, Marie-Cecile Maniere, and Agnes Bloch-Zupan -- Protocols for genetic and epigenetic studies of rare diseases affecting dental tissues / Bruna Rabelo Amorim, Pollyanna Almeida Costa dos Santos, Caroline Lourenco de Lima, Denise Carleto Andia, Juliana Forte Mazzeu, and Ana Carolina Acevedo -- Protocols, Methods, and tools for genome-wide association studies (GWAS) of dental traits / Cary S. Agler, Dmitry Shungin, Andrea G. Ferreira Zandona, Paige Schmadeke, Patricia V. Basta, Jason Luo, John Cantrell, Thomas D. Pahel Jr., Beau D. Meyer, John R. Shaffer, Arne S. Schaefer, Kari E. North, and Kimon Divaris -- Measurement of early childhood oral health for research purposes: dental caries experience and developmental defects of the enamel in the primary dentition |
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Jeannie Ginnis, Andrea G. Ferreira Zandona, Gary D. Slade, John Cantrell, Mikafui E. Antonio, Bhavna T. Pahel, Beau D. Meyer, Poojan Shrestha, Miguel A. Simancas-Pallares, Ashwini R. Joshi, and Kimon Divaris -- Supragingival biofilm in early childhood caries : clinical and laboratory protocols and bioinformatics pipelines supporting metagenomics, metatranscriptomics, and metabolomics studies of the oral microbiome / Kimon Divaris, Dmitry Shungin, Adaris Rodrıþguez-Cortes, Patricia V. Basta, Jeff Roach, Hunyong Cho, Di Wu, Andrea G. Ferreira Zandona, Jeannie Ginnis, Sivapriya Ramamoorthy, Jason M. Kinchen, Jakub Kwintkiewicz, Natasha Butz, Apoena A. Ribeiro, and M. Andrea Azcarate-Peril -- Saliva and gingival crevicular fluid (GCF) collection for biomarker screening / Petros Papagerakis, Li Zheng, Doohak Kim, Raed Said, Amber A. Ehlert, Kevin K.M. Chung, and Silvana Papagerakis |
| Summary |
This volume provides methods and approaches to study genetic and environmental regulatory controls on odontogenesis. Chapters guide readers through protocols for isolation and characterization of both epithelial and mesenchymal dental cells, methods on isolation, phenotypic characterization, expansion, differentiation, immunofluorescence, in situ hybridization, immunohistochemistry, imaging protocols, rodent dental fluorosis model, 3D assessment of crown size, dental diseases models, next generation sequencing, genetic and epigenetic studies, genome-wide association studies as well as clinical protocols for measurement of early childhood caries and saliva, and supragingival fluids and biofilm collection and subsequent analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Teeth -- Growth -- Laboratory manuals
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Odontogenesis
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Tooth -- growth & development
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Teeth -- Growth
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| Genre/Form |
Electronic books
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Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Papagerakis, Petros, editor
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| ISBN |
9781493990122 |
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1493990128 |
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