Description |
1 online resource (x, 377 pages) : illustrations |
Series |
Methods in molecular biology ; v. 40 |
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Methods in molecular biology (Clifton, N.J.) ; v. 40
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Contents |
Noncovalent forces important to the conformational stability of protein structures / Kenneth P. Murphy -- Degradative covalent reactions important to protein stability / David B. Volkin, Henryk Mach, C. Russell Middaugh -- Fluorescence spectroscopy / Catherine A. Royer -- Ultraviolet absorption spectroscopy / Henryk Mach, David B. Volkin, Carl J. Burke, C. Russell Middaugh -- Circular dichroism / Kunihiro Kuwajima -- Infrared spectroscopy / C. Russell Middaugh, Henryk Mach, James A. Ryan, Gautam Sanyal, David B. Volkin -- Identifying sites of posttranslational modifications in proteins via HPLC peptide mapping / Kenneth R. Williams, Kathryn L. Stone -- Urea and guanidine hydrochloride denaturation curves / Bret A. Shirley -- Differential scanning calorimetry / Ernesto Freire -- Disulfide bonds in protein folding and stability / Nigel Darby, Thomas E. Creighton -- Solvent stabilization of protein structure / Serge N. Timasheff -- Site-directed mutagenesis to study protein folding and stability / Philip N. Bryan -- Hydrogen exchange techniques / J. Martin Scholtz, Andrew D. Robertson -- Protein folding kinetics / Thomas Kiefhaber -- Molten globules / Anthony L. Fink -- Chaperonin-assisted protein folding of the enzyme rhodanese by GroEL/GroES / Paul M. Horowitz |
Summary |
In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners |
Analysis |
biochemie |
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biochemistry |
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levenswetenschappen |
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life sciences |
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Biology (General) |
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Biologie (algemeen) |
Bibliography |
Includes bibliographical references and index |
Notes |
English |
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Print version record |
Subject |
Protein folding.
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Proteins -- Conformation.
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Protein Conformation
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Protein Folding
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Protein folding
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Proteins -- Conformation
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Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Shirley, Bret A.
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ISBN |
9781592595273 |
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1592595278 |
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1280836490 |
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9781280836497 |
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9786610836499 |
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6610836493 |
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