Description |
xxiii, 242 pages : illustrations ; 24 cm |
Series |
Practical approach series. ; 140 |
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Practical approach series.
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Contents |
1. Historical introduction / J. M. Gershoni -- 2. Introduction and basic set-up for protein transfer / Bonnie S. Dunbar -- 3. Protein blotting and immunoblotting using nitrocellulose membranes / Antonio De Maio -- 4. Protein blotting using polyvinylidene fluoride membranes / Michael A. Mansfield -- 5. Protein blotting using semi-dry electrophoretic transfer equipment / Gunilla Jacobson -- 6. Transfer and blocking conditions in immunoblotting / A. Van Dam -- 7. Sample preparation for protein electrophoresis and transfer / Vaughan H. Lee and Bonnie S. Dunbar -- 8. Strategy for purification of organelles from parasites: protein blotting as one of the initial steps / David W. Sammons -- 9. Radiometric methods for detection in blots / R. A. Laskey -- 10. Bioluminescence-enhanced detection systems in protein blotting / Reinhard Erich Geiger -- 11. Chemiluminescent detection systems for protein blotting / Ian Durrant and Sue Fowler |
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12. Detection and characterization of glycoprotein carbohydrate chains after electrophoretic separation / Tatsuro Irimura and Hiroto Kawashima -- 13. DNA binding of immobilized proteins / Constantin N. Flytzanis -- 14. Use of peptide ligands for the detection of binding proteins by Western blotting / Paul Hossenlopp and Michel Binoux -- 15. Use of blotted proteins as immunogens / Boguslaw Szewczyk and David R. Harper -- 16. Amino acid sequence analysis of blotted proteins / Richard G. Cook -- 17. Renaturative catalytic blotting of enzyme proteins / Jia-Shi Zhu and Gary M. Gray |
Analysis |
Proteins Chemical analysis |
Bibliography |
Includes bibliographical references and index |
Subject |
Western immunoblotting -- Laboratory manuals.
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Western immunoblotting.
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Proteins -- Analysis.
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Western immunoblotting -- Laboratory manuals.
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Blotting, Western.
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Proteins -- analysis.
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Author |
Dunbar, Bonnie S.
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LC no. |
93042043 |
ISBN |
0199634378 (paperback) |
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0199634386 (hbk.) |
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