Limit search to available items
Book Cover
Book

Title Protein blotting : a practical approach / edited by Bonnie S. Dunbar
Published Oxford ; New York : IRL Press at Oxford University Press, [1994]
©1994

Copies

Location Call no. Vol. Availability
 W'PONDS  574.19245 Dun/Pba  AVAILABLE
Description xxiii, 242 pages : illustrations ; 24 cm
Series Practical approach series. ; 140
Practical approach series.
Contents 1. Historical introduction / J. M. Gershoni -- 2. Introduction and basic set-up for protein transfer / Bonnie S. Dunbar -- 3. Protein blotting and immunoblotting using nitrocellulose membranes / Antonio De Maio -- 4. Protein blotting using polyvinylidene fluoride membranes / Michael A. Mansfield -- 5. Protein blotting using semi-dry electrophoretic transfer equipment / Gunilla Jacobson -- 6. Transfer and blocking conditions in immunoblotting / A. Van Dam -- 7. Sample preparation for protein electrophoresis and transfer / Vaughan H. Lee and Bonnie S. Dunbar -- 8. Strategy for purification of organelles from parasites: protein blotting as one of the initial steps / David W. Sammons -- 9. Radiometric methods for detection in blots / R. A. Laskey -- 10. Bioluminescence-enhanced detection systems in protein blotting / Reinhard Erich Geiger -- 11. Chemiluminescent detection systems for protein blotting / Ian Durrant and Sue Fowler
12. Detection and characterization of glycoprotein carbohydrate chains after electrophoretic separation / Tatsuro Irimura and Hiroto Kawashima -- 13. DNA binding of immobilized proteins / Constantin N. Flytzanis -- 14. Use of peptide ligands for the detection of binding proteins by Western blotting / Paul Hossenlopp and Michel Binoux -- 15. Use of blotted proteins as immunogens / Boguslaw Szewczyk and David R. Harper -- 16. Amino acid sequence analysis of blotted proteins / Richard G. Cook -- 17. Renaturative catalytic blotting of enzyme proteins / Jia-Shi Zhu and Gary M. Gray
Analysis Proteins Chemical analysis
Bibliography Includes bibliographical references and index
Subject Western immunoblotting -- Laboratory manuals.
Western immunoblotting.
Proteins -- Analysis.
Western immunoblotting -- Laboratory manuals.
Blotting, Western.
Proteins -- analysis.
Author Dunbar, Bonnie S.
LC no. 93042043
ISBN 0199634378 (paperback)
0199634386 (hbk.)