| Description |
pages cm |
| Series |
Practical approach series ; v. <148, 149 > |
|
The practical approach series ; 149 |
|
Practical approach series ; v. <148, 149 >
|
|
Practical approach series ; 149
|
| Contents |
v. 1. Core techniques -- v. 2. Expression systems |
| Summary |
This book is the second of a revised set of four Practical Approach volumes on DNA Cloning which cover the major experimental techniques in use in modern molecular biology laboratories. The second volume, Expression Systems, is written by established researchers in the field. It provides essential background information and full details of current laboratory procedures for the expression of cDNA clones in E. coli, lambda-phage, yeast, and baculovirus expression systems, together with methods for screening libraries, isolation of proteins on the basis of protein-protein interactions, purification of the desired protein(s), and the generation of monoclonal and polyclonal antibodies. Each chapter contains key literature citations to acquaint and inform the reader, together with background information, up-to-date protocols, advice, hints, tips, and troubleshooting comments |
| Analysis |
DNA Synthesis |
| Notes |
Library holds v. 2 only |
| Bibliography |
Includes bibliographical references and indexes |
| Subject |
Molecular cloning -- Laboratory manuals.
|
|
Molecular cloning.
|
|
Recombinant DNA -- Laboratory manuals.
|
|
Recombinant DNA.
|
| Author |
Glover, David M.
|
|
Hames, B. D.
|
| LC no. |
94026740 |
| ISBN |
0199634769 (v. 1 : pbk.) |
|
0199634777 (v. 1) |
|
0199634785 (v. 2 : pbk.) |
|
0199634793 (v. 2) |
|
