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Title Proteins / edited by John M. Walker
Published Clifton, N.J. : Humana Press, ©1984

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Description 1 online resource (xii, 365 pages) : illustrations
Series Methods in molecular biology ; v. 1
Biological methods
Biological methods.
Methods in molecular biology (Clifton, N.J.) ; v. 1
Contents The Lowry method for protein quantitation / Jaap H. Waterborg, Harry R. Matthews -- Determination of protein molecular weights by gel permeation high pressure liquid chromatography / E.L. Mayes -- Immunoaffinity purification of protein antigens / E.L. Mayes -- Electrophoretic and chromatographic separation of peptides on paper / E.L. Mayes -- Peptide mapping by reverse-phase high pressure liquid chromatography / E.L. Mayes -- SDS polyacrylamide gel electrophoresis of proteins / B.J. Smith -- Gradient SDS polyacrylamide gel electrophoresis / John M. Walker -- Acetic acid-urea polyacrylamide gel electrophoresis of proteins / B.J. Smith -- The in vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis / Jeffrey W. Pollard -- Two-dimensional polyacrylamide gel electrophoresis of proteins / Jeffrey W. Pollard -- Starch gel electrophoresis of proteins / Graham B. Divall -- Isoelectric focusing in ultrathin polyacrylamide gels / Graham B. Divall -- High-sensitivity silver staining of proteins following polyacrylamide gel electrophoresis / Keith Gooderham -- Quantification of proteins on polyacrylamide gels (nonradioactive) / B.J. Smith -- Computer analysis of gel scans / Harry R. Matthews -- Drying gels / Bryan J. Smith -- Fluorography of polyacrylamide gels containing tritium / Jaap H. Waterborg, Harry R. Matthews -- Recovery of proteins from dried polyacrylamide gels after fluorography / Jaap H. Waterborg, Harry R. Matthews -- The electrophoretic elution of proteins from polyacrylamide gels / Jaap H. Waterborg, Harry R. Matthews -- Transfer techniques in protein blotting / Keith Gooderham -- Peptide mapping by thin-layer chromatography and high voltage electrophoresis / Keith Gooderham -- In situ peptide mapping of proteins following polyacrylamide gel electrophoresis / Keith Gooderham -- The Dansyl method for identifying N-terminal amino acids / John M. Walker -- The Dansyl-Edman method for peptide sequencing / John M. Walker -- Microsequencing of peptides and proteins with 4-N, N-dimethylaminoazobenzene-4'-isothiocyanate / Brigitte Wittmann-Liebold, Makoto Kimura -- Manual Edman degradation of proteins and peptides / Per Klemm -- Carboxy-terminal sequence determination of proteins and peptides with carboxypeptidase Y / Per Klemm -- Immunization and fusion protocols for hybridoma production / J.N. Wood -- Immunofluorescence and immunoperoxidase screening of hybridomas / J.N. Wood -- Solid-phase screening of monoclonal antibodies / J.N. Wood -- Subclass analysis and purification of monoclonal antibodies / J.N. Wood -- The production of antisera / G.S. Bailey -- Immunodiffusion in gels / G.S. Bailey -- Crossed immunoelectrophoresis / Graham B. Divall -- Rocket immunoelectrophoresis / John M. Walker -- Radioiodination of proteins / G.S. Bailey -- Radioimmunoassay / G.S. Bailey -- Enzyme-linked immunosorbant assay (ELISA) / Wim Gaastra
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL
Description based on print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
Subject Proteins -- Analysis -- Methodology
Molecular biology -- Research -- Methodology
Proteins -- analysis
Molecular Biology -- methods
Molecular biology -- Research -- Methodology
Proteins -- Analysis -- Methodology
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Walker, John M., 1948-
LC no. 92129967
ISBN 9781592594887
1592594883
0896030628
9780896030626