Description |
1 online resource (xii, 365 pages) : illustrations |
Series |
Methods in molecular biology ; v. 1 |
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Biological methods |
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Biological methods.
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Methods in molecular biology (Clifton, N.J.) ; v. 1
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Contents |
The Lowry method for protein quantitation / Jaap H. Waterborg, Harry R. Matthews -- Determination of protein molecular weights by gel permeation high pressure liquid chromatography / E.L. Mayes -- Immunoaffinity purification of protein antigens / E.L. Mayes -- Electrophoretic and chromatographic separation of peptides on paper / E.L. Mayes -- Peptide mapping by reverse-phase high pressure liquid chromatography / E.L. Mayes -- SDS polyacrylamide gel electrophoresis of proteins / B.J. Smith -- Gradient SDS polyacrylamide gel electrophoresis / John M. Walker -- Acetic acid-urea polyacrylamide gel electrophoresis of proteins / B.J. Smith -- The in vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis / Jeffrey W. Pollard -- Two-dimensional polyacrylamide gel electrophoresis of proteins / Jeffrey W. Pollard -- Starch gel electrophoresis of proteins / Graham B. Divall -- Isoelectric focusing in ultrathin polyacrylamide gels / Graham B. Divall -- High-sensitivity silver staining of proteins following polyacrylamide gel electrophoresis / Keith Gooderham -- Quantification of proteins on polyacrylamide gels (nonradioactive) / B.J. Smith -- Computer analysis of gel scans / Harry R. Matthews -- Drying gels / Bryan J. Smith -- Fluorography of polyacrylamide gels containing tritium / Jaap H. Waterborg, Harry R. Matthews -- Recovery of proteins from dried polyacrylamide gels after fluorography / Jaap H. Waterborg, Harry R. Matthews -- The electrophoretic elution of proteins from polyacrylamide gels / Jaap H. Waterborg, Harry R. Matthews -- Transfer techniques in protein blotting / Keith Gooderham -- Peptide mapping by thin-layer chromatography and high voltage electrophoresis / Keith Gooderham -- In situ peptide mapping of proteins following polyacrylamide gel electrophoresis / Keith Gooderham -- The Dansyl method for identifying N-terminal amino acids / John M. Walker -- The Dansyl-Edman method for peptide sequencing / John M. Walker -- Microsequencing of peptides and proteins with 4-N, N-dimethylaminoazobenzene-4'-isothiocyanate / Brigitte Wittmann-Liebold, Makoto Kimura -- Manual Edman degradation of proteins and peptides / Per Klemm -- Carboxy-terminal sequence determination of proteins and peptides with carboxypeptidase Y / Per Klemm -- Immunization and fusion protocols for hybridoma production / J.N. Wood -- Immunofluorescence and immunoperoxidase screening of hybridomas / J.N. Wood -- Solid-phase screening of monoclonal antibodies / J.N. Wood -- Subclass analysis and purification of monoclonal antibodies / J.N. Wood -- The production of antisera / G.S. Bailey -- Immunodiffusion in gels / G.S. Bailey -- Crossed immunoelectrophoresis / Graham B. Divall -- Rocket immunoelectrophoresis / John M. Walker -- Radioiodination of proteins / G.S. Bailey -- Radioimmunoassay / G.S. Bailey -- Enzyme-linked immunosorbant assay (ELISA) / Wim Gaastra |
Bibliography |
Includes bibliographical references and index |
Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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Description based on print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
Subject |
Proteins -- Analysis -- Methodology
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Molecular biology -- Research -- Methodology
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Proteins -- analysis
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Molecular Biology -- methods
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Molecular biology -- Research -- Methodology
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Proteins -- Analysis -- Methodology
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Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Walker, John M., 1948-
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LC no. |
92129967 |
ISBN |
9781592594887 |
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1592594883 |
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0896030628 |
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9780896030626 |
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