In vitro synthesis of proteins in bacterial extracts -- Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation -- Yeast two-hybrid screen -- UV cross-linking of interacting RNA and protein in cultured cells -- Analysis of RNA-protein interactions by cell mixing -- General protein-protein cross-linking -- Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans -- PAR-CLIP (phoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins -- Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system -- Dissecting a known RNA-protein interaction using a yeast three-hybrid system -- Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system
