| Description |
1 online resource (xi, 208 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; v. 1219 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1219. 1064-3745
|
| Contents |
Caspase-3 activation is a critical determinant of genotoxic stress-induced apoptosis / Gaurav S. Choudhary, Sayer Al-harbi, and Alexandru Almasan -- Flow cytometry enumeration of apoptotic cancer cells by apoptotic rate / David Diaz, Alfredo Prieto, Eduardo Reyes, Hugo Barcenilla, Joge Monserrat, Melchor Alvarez-Mon -- "Multiplexed viability, cytotoxicity, and caspase activity assays" / Andrew L. Niles and Terry L. Riss -- Multiplexed method for kinetic measurements of apoptosis and proliferation using live-content imaging / Katherine Artymovich and Daniel M. Appledorn -- Detection of end-stage apoptosis by ApopTag® TUNEL technique / Chandra Mohan, Kevin Long, Manpreet Mutneja, Jun Ma -- Detection and quantification of apoptosis in primary cells using Taqman® protein assay / Christina Pfister, Heike Pfrommer, Marcos S. Tatagiba, Florian Roser -- Detection of p53 protein aggregation in cancer cell lines and tumor samples / Yang Yang-Hartwich, Jamie Bingham, Francssca Garofalo, Ayesha B. Alvero, Gil Mor -- Detection of p53 protein transcriptional activity by chromatin immunoprecipitation / Yang Yang-Hartwichm=, Emikly Romanoff, Jamie Bingham, Ayesha B. Alvero, Gil Mor -- Homogeneous, bioluminescent proteasome assays / Martha A. O'Brien, Richard A. Moravec, Terry L. Riss, Robert F. Bulleit -- Laser capture microdissection for gene expression analysis / Mallikarjun Bidarimath, Andrew K. Edwards, and Chandrakant Tayade -- Using the Peggy simple western system for fine needle aspirate analysis / Erik T. Gentalen and John M. Proctor -- Analysis of autophagosome formation using lentiviral biosensors for live fluorescent cellular imaging / Kevin Long, Chandra Mohan, Janet Anderl, Karyn Huryn-Selvar, Haizhen Liu, Kevin Su, Mark Santos, :Matthew Hsu, Lucas Armstrong, Jun Ma -- Optical imaging of ovarian cancer using HER-2 affibody conjugated nanoparticles / Minati Satpathy, Rafal Zielinski, Ilya Lyakhov, Lily Yang -- Measuring cardiac autophagic flux in vitro and in vivo / Michael A. Gurney, Ghengqun Huang, Jennifer M. Ramil, Nandini Ravindran, Allen M. Andres, Jon Sin, Phyllis-Jeanm Linton, Roberta A. Gottlieb -- PET imaging for tyrosine kinase inhibitor (TKI) biodistribution in mice / Hiroshi Fushiki, Yoshihiro Murakami, Sosuke Miyoshi, Shintaro Nishimura |
| Summary |
In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway. Chapters focus on assays developed to detect its activation not only in vitro but also in vivo, optimized multiplex analysis, medium- to high-throughput screens, and the cellular process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Apoptosis and Cancer: Methods and Protocols, Second Edition aids scientists as a stand-alone resource for the execution and analysis of the described protocols and as a reference for the study and detection of apoptosis within and outside the area of cancer research |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed October 15, 2014) |
| Subject |
Carcinogenesis.
|
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Apoptosis.
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Cancer cells.
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Tumors -- Growth -- Regulation
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Neoplasms -- etiology
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Apoptosis
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Apoptosis -- physiology
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Caspases
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Neoplasms -- physiopathology
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Carcinogenesis
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Apoptosis
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Cancer cells
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Carcinogenesis
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Apoptosis
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Biologisches Verfahren
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Krebs Medizin
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Molekularbiologie
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
|
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Aufsatzsammlung.
|
| Form |
Electronic book
|
| Author |
Mor, Gil, editor.
|
|
Alvero, Ayesha B., editor.
|
| ISBN |
9781493916610 |
|
1493916610 |
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1493916602 |
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9781493916603 |
|
