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E-book

Title Transposons and retrotransposons : methods and protocols / edited by Jose L. Garcia-Pérez
Published New York, NY : Humana Press, 2016

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Description 1 online resource (xii, 434 pages) : illustrations (some color)
Series Methods in Molecular Biology, 1064-3745 ; 1400
Methods in molecular biology (Clifton, N.J.) ; v. 1400. 1064-3745
Contents Study of transposable elements and their genomic impact / Martin Muñoz-Lopez [and others] -- Bacterial group II introns : identification and mobility assay / Nicolás Toro [and others] -- In silico methods to identify exapted transposable element families / LeeAnn Ramsay and Guillaume Bourque -- Retrotransposon capture sequencing (RC-Seq) : a targeted, high-throughput approach to resolve somatic L1 retrotransposition in humans / Francisco J. Sanchez-Luque, Sandra R. Richardson, and Geoffrey J. Faulkner -- Long interspersed element sequencing (L1-Seq) : a method to identify somatic LINE-1 insertions in the human genome / Tara T. Doucet and Haig H. Kazazian Jr. -- Combining amplification typing of L1 active subfamilies (ATLAS) with high-throughput sequencing / Raheleh Rahbari and Richard M. Badge -- RNA-Seq analysis to measure the expression of SINE retroelements / Ángel Carlos Román, Antonio Morales-Hernández, and Pedro M. Fernández-Salguero -- Qualitative and quantitative assays of transposition and homologous recombination of the retrotransposon Tf1 in Schizosaccharomyces pombe / Maya Sangesland [and others] -- LINE retrotransposition assays in Saccharomyces cerevisiae / Axel V. Horn and Jeffrey S. Han -- LINE-1 cultured cell retrotransposition assay / Huira C. Kopera [and others] -- L1 retrotransposition in neural progenitor cells / Alysson R. Muotri -- Characterization of engineered L1 retrotransposition events : the recovery method / David Cano [and others] -- SINE retrotransposition : evaluation of alu activity and recovery of de novo inserts / Catherine Ade and Astrid M. Roy-Engel -- Engineered SVA trans-mobilization assay / Anja Bock and Gerald G. Schumann -- Detection of LINE-1 RNAs by northern blot / Prescott Deininger and Victoria P. Belancio -- Monitoring long interspersed nuclear element 1 expression during mouse embryonic stem cell differentiation / Maxime Bodak and Constance Ciaudo -- Immunodetection of human LINE-1 expression in cultured cells and human tissues / Reema Sharma [and others] -- Cellular localization of engineered human LINE-1 RNA and proteins / Aurélien J. Doucet, Eugénia Basyuk, and Nicolas Gilbert -- Purification of L1-ribonucleoprotein particles (L1-RNPs) from cultured human cells / Prabhat K. Mandal and Haig H. Kazazian Jr. -- Characterization of L1-ribonucleoprotein particles / Martin S. Taylor [and others] -- LEAP : L1 element amplification protocol / Huira C. Kopera [and others] -- Biochemical approaches to study LINE-1 reverse transcriptase activity in vitro / Sébastien Viollet, Aurélien J. Doucet, and Gaël Cristofari -- Methylated DNA immunoprecipitation analysis of mammalian endogenous retroviruses / Rita Rebollo and Dixie L. Mager -- Profiling DNA methylation and hydroxymethylation at retrotransposable elements / Lorenzo de la Rica, Jatinder S. Stanley, and Miguel R. Branco -- Large-scale functional screen to identify epigenetic repressors of retrotransposon expression / Gabriela Ecco, Helen M. Rowe, and Didier Trono -- Reprogramming of human fibroblasts to induced pluripotent stem cells with sleeping beauty transposon-based stable gene delivery / Attila Sebe and Zoltán Ivics
Notes Includes index
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed February 23, 2016)
Subject Transposons -- Laboratory manuals
DNA Transposable Elements
Retroelements
Medical genetics.
Medical -- Genetics.
Transposons
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author García Pérez, José L., editor
ISBN 9781493933723
1493933728
1493933701
9781493933709