Description |
1 online resource (streaming video file) (581 secs.) |
Series |
Biology |
Summary |
We describe a protocol for visualization of insulin exocytosis in intact islets using pHluorin, a pH-sensitive green fluorescent protein. Isolated islets are infected with adenovirus encoding pHluorin coupled to the vesicle cargo neuropeptide Y. This allows for the detection of insulin granule fusion events by confocal microscopy |
Audience |
For undergraduate, graduate, and professional students |
Notes |
English |
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Description based on online resource; title from resource description page |
Subject |
Biology
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Form |
Streaming audio
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