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Book

Title Neuroscience methods : a guide for advanced students / edited by Rosemary Martin
Published Amsterdam : Harwood Academic Publishers, [1997]
©1997

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Location Call no. Vol. Availability
 W'PONDS  612.8 Mar/Nma  AVAILABLE
Description xii, 260 pages : illustrations ; 26 cm
Contents Sect. 1. In Vitro Preparations. Ch. 1. Slices of brain tissue / C. Stricker. Ch. 2. The brain in a dish: How to study acutely dissociated and cultured neurons / J. M. Bekkers. Ch. 3. Painting optimal bilayers for recording ion channels / J. I. Kourie -- Sect. 2. Electrophysiological Techniques. Ch. 4. Extracellular single neuronal recordings in the whole animal / T. R. Vidyasagar. Ch. 5. Equipment for measurement of membrane potentials and currents, with special reference to sharp microelectrodes / R. L. Martin. Ch. 6. Sucking up to cells: The patch-clamp technique in neuroscience / J. M. Bekkers. Ch. 7. Analysis of single channel recordings / D. Laver -- Sect. 3. Human Electrophysiology. Ch. 8. Recording electromyographic activity (emg) from single motor units in human subjects / V. G. Macefield. Ch. 9. Intraneural recording from single nerve fibers in awake human subjects / V. G. Macefield. Ch. 10. Somatosensory cortical evoked potentials recorded from human subjects / V. G. Macefield -- Sect. 4. Application of Drugs. Ch. 11. Very fast drug application to dissociated cells and membrane patches / J. Clements. Ch. 12. Microelectrophoresis and pressure ejection methods / G. Lacey -- Sect. 5. Measurement of Ion Concentrations. Ch. 13. Ion-selective microelectrodes / A. I. Cowan. Ch. 14. Fluorescent dyes for measurement of intracellular ion concentrations / N. Mahanty -- Sect. 6. In Vivo Techniques and Preparations. Ch. 15. Stereotaxic placement of probes in neurobiology / S. P. Perrett. Ch. 16. In vivo microdialysis / A. J. Lawrence. Ch. 17. Electroretinography / A. Leon -- Sect. 7. Histochemical Techniques. Ch. 18. Anatomical tracing of neuronal connections / L. R. Marotte. Ch. 19. Immunohistochemistry / P. Cooper. Ch. 20. High resolution autoradiography / S. Oleskevich -- Sect. 8. Biochemical Techniques. Ch. 21. Membrane fractionation / P. R. Junankar. Ch. 22. Chromatographic techniques for protein separation and purification / G. M. de Plater -- Sect. 9. Production of Antibodies. Ch. 23. Production and uses of polyclonal antibodies / M. F. Crouch. Ch. 24. Production and uses of monoclonal antibodies / W. D. Phillips -- Sect. 10. Blotting and Hybridization Techniques. Ch. 25. Immunoblotting (Western blotting) / S. Johanson. Ch. 26. Southern and Northern blotting / S. Howitt. Ch. 27. Membrane and in situ hybridization: Principles and pitfalls / B. Key -- Sect. 11. Expression Systems. Ch. 28. Cellular expression of cloned and mutated ion channels / P. R. Schofield. Ch. 29. Transfection in cell cultures / W. D. Phillips -- Sect. 12. Genetic Techniques. Ch. 30. Reverse transcription polymerase chain reaction / M. Vidovic. Ch. 31. Differential display of gene expression / F. M. Freeman. Ch. 32. Inhibition of gene expression with antisense oligonucleotides / P. M. Pilowsky. Ch. 33. Creation of transgenic mice / P. G. Noakes -- Sect. 13. Neural Grafting. Ch. 34. Neural grafting / L. J. Reece
Bibliography Includes bibliographical references and indexes
Subject Central nervous system -- Research.
Neurology -- Technique.
Neurophysiology -- Research -- Methodology.
Neurophysiology -- Research -- Technique.
Neurosciences -- Methodology.
Neurosciences -- Research -- Methodology.
Neurosciences -- Research -- Technique.
Neurosciences -- Technique.
Nervous System.
Neurosciences -- methods.
Author Martin, Rosemary (Rosemary L.)
LC no. 99521816
ISBN 9057022443 (hard cover)
9057022451 (paperback)