| Description |
1 online resource (xiv, 335 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; 1426 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1426
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| Contents |
Evolution and epidemiology of chikungunya virus / Giada Rossini, Maria Paola Landini, and Vittorio Sambri -- Molecular epidemiology of chikungunya virus by sequencing / Ravindran Thayan [and others] -- Advanced genetic methodologies in tracking evolution and spread of chikungunya virus / Hapuarachchige Chanditha Hapuarachchi and Kim-Sung Lee -- Synthetic peptide-based antibody detection for diagnosis of chikungunya infection with and without neurological complications / Rajpal S. Kashyap [and others] -- Expression and purification of E2 glycoprotein from insect cells (Sf9) for use in serology / Chong Long Chua, I-Ching Sam, and Yoke Fun Chan -- Diagnostic methods for CHIKV based on serological tools / Paolo Gaibani, Maria Poala Landini, and Vittorio Sambri -- Utilization and assessment of throat swab and urine specimens for diagnosis of chikungunya virus infection / Chandrashekhar G. Raut, H. Hanumaiah, and Wrunda C. Raut -- Propagation of chikungunya virus using mosquito cells / Swee Kim Ang, Shirley Lam, and Justin Jang Hann Chu -- Infectious viral quantification of chikungunya virus-virus plaque assay / Parveen Kaur, Regina Ching Hua Lee, and Justin Jang Hann Chu -- Detection and quantification of chikungunya virus by real-time RT-PCR assay / Seok Mui Wang [and others] -- Chikungunya virus infection of aedes mosquitoes / Hui Vern Wong [and others] -- Analysis of CHIKV in mosquitoes infected via artificial blood meal / Jeremy P. Ledermann and Ann M. Powers -- Chikungunya virus growth and fluorescent labeling : detection of chikungunya virus by immunofluorescence assay / Meng Ling Moi and Tomohiko Takasaki -- Virus isolation and preparation of sucrose-banded chikungunya virus samples for transmission electron microscopy / Chang-Kweng Lim -- Viral-host protein interaction studies using yeast two-hybrid screening method / Namrata Dudha and Sanjay Gupta -- Application of GelC-MS/MS to proteomic profiling of chikungunya virus infection : preparation of peptides for analysis / Atchara Paemanee [and others] -- Bioinformatics based approaches to study virus-host interactions during chikungunya virus infection / Sreejith Rajasekharan and Sanjay Gupta -- T-cell epitope prediction of chikungunya virus / Christine Loan Ping Eng, Tin Wee Tan, and Joo Chuan Tong -- Mouse models of chikungunya virus / Lara J. Herrero [and others] -- Generation of mouse monoclonal antibodies specific to chikungunya virus using ClonaCell-HY hybridoma cloning kit / Chow Wenn Yew and Yee Joo Tan -- Immunohistochemical detection of chikungunya virus antigens in formalin-fixed and paraffin-embedded tissues / Chun Wei Chiam [and others] -- Antiviral strategies against chikungunya virus / Rana Abdelnabi, Johan Neyts, and Leen Delang -- Real-time cell analyzing assay for identification of novel antiviral compounds against chikungunya virus / Keivan Zandi -- Using bicistronic baculovirus expression vector system to screen the compounds that interfere with the infection of chikungunya virus / Szu-Cheng Kuo [and others] -- Neutralization assay for chikungunya virus infection : plaque reduction neutralization test / Nor Azila Muhammad Azami, Meng Ling Moi, and Tomohiko Takasaki -- Reverse genetics approaches for chikungunya virus / Patchara Phuektes and Justin Jang Hann Chu -- Production of chikungunya virus-like particles and subunit vaccines in insect cells / Stefan W. Metz and Gorben P. Pijlman -- Protocols for developing novel chikungunya virus DNA vaccines / Christopher Chung [and others] |
| Summary |
This volume covers multidisciplinary approaches on various aspects of Chikungunya Virus (CHIKV) research that was brought together from leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, bioinformatics, and proteomics approaches in cellular response studies. Chikungunya Virus: Methods and Protocols also discusses methods in immunology and animal model studies, as well as different strategies of antivirals and vaccine development for therapeutics against CHIKV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Chikungunya Virus: Methods and Protocols is a useful and comprehensive resource for CHIKV researchers and anyone interested in learning more about the Chikungunya Virus |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF of title page (Springer Protocols, viewed May 31, 2016) |
| Subject |
Chikungunya -- Laboratory manuals
|
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Chikungunya virus
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Chikungunya Fever
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Medical microbiology & virology.
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Infectious & contagious diseases.
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Medical -- Microbiology.
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Medical -- Infectious Diseases.
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Chikungunya
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
|
| Form |
Electronic book
|
| Author |
Chu, Justin Jang Hann.
|
|
Ang, Swee Kim
|
| LC no. |
2016935402 |
| ISBN |
9781493936182 |
|
1493936182 |
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1493936166 |
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9781493936168 |
|
