| Description |
1 online resource (xxix, 926 pages) : illustrations |
| Series |
Methods in enzymology ; v. 171 |
|
Methods in enzymology ; v. 171
|
| Contents |
Gentle and fast transmembrane reconstitution of membrane proteins / Norbert A. Dencher, Sidney Fleischer and Becca Fleischer -- Ion carriers in planar bilayers: Relaxation techniques and noise analysis / R. Benz ... [et al.] -- Ion interactions at membranous polypeptide sites using nuclear magnetic resonance: Determining rate and binding constants and site locations / Dan W. Urry ... [et al.] -- Measuring electrostatic potentials adjacent to membranes / David Cafiso ... [et al.] -- Determination of surface potential of biological membranes / Hagai Rottenberg, Sidney Fleischer and Becca Fleischer -- Lipid coumarin dye as a probe of interfacial electrical potential in biomembranes / Peter Fromherz, Sidney Fleischer and Becca Fleischer -- Modulation of membrane protein function by surface potential / Reinhard Krämer, Sidney Fleischer and Becca Fleischer -- Use of nonequilibrium thermodynamics in the analysis of transport: General flow-force relationships and the linear domain / Daniela Pietrobon ... [et al.] -- Cell isolation techniques: Use of enzymes and chelators / Miguel J.M. Lewin ... [et al.] -- Cell separation by gradient centrifugation methods / Thomas G. Pretlow ... [et al.] -- Cell separation by elutriation: Major and minor cell types from complex tissues / Martin J. Sanders ... [et al.] -- Cell separation using velocity sedimentation at unit gravity and buoyant density centrifugation / John Wells, Sidney Fleischer and Becca Fleischer -- Separation of cell populations by free-flow electrophoresis / Hans- G. Heidrich ... [et al.] |
|
Separation of cells and cell organelles by partition in aqueous polymer two-phase systems / Per-åke Albertsson, Sidney Fleischer and Becca Fleischer -- Flow cytometry: Rapid isolation and analysis of single cells / Bruce D. Jensen ... [et al.] -- Use of cell-specific monoclonal antibodies to isolate renal epithelia / Arlyn Garcia-Perez ... [et al.] -- Pancreatic acini as second messenger models in exocrine secretion / Jerry D. Gardner ... [et al.] -- Ascites cell preparation: Strains, caveats / Rose M. Johnstone ... [et al.] -- Direct current electrical measurement in epithelia: Steady-state and transient analysis / Warren S. Rehm ... [et al.] -- Impedance analysis in tight epithelia / Chris Clausen, Sidney Fleischer and Becca Fleischer -- Electrical impedance analysis of leaky epithelia: Theory, techniques, and leak artifact problems / L.G.M. Gordon ... [et al.] -- Patch-clamp experiments in epithelia: Activation by hormones or neurotransmitters / Ole H. Petersen, Sidney Fleischer and Becca Fleischer -- Ionic permeation mechanisms in epithelia: Biionic potentials, dilution potentials, conductances, and streaming potentials / Peter H. Barry, Sidney Fleischer and Becca Fleischer -- Use of ionophores in epithelia: Characterizing membrane properties / Simon A. Lewis ... [et al.] -- Cultures as epithelial models: Porous-bottom culture dishes for studying transport and differentiation / Joseph S. Handler ... [et al.] -- Volume regulation in epithelia: Experimental approaches / Anthony D.C. Macknight ... [et al.] -- Scanning electrode localization of transport pathways in epithelial tissues / J. Kevin Foskett ... [et al.] -- Experimental control of intracellular environment / Peter F. Baker ... [et al.] -- Implantation of Isolated Carriers and receptors into living cells by Sendai virus envelope-mediated fusion / Abraham Loyter ... [et al.] -- Resonance energy transfer microscopy: Visual colocalization of fluorescent lipid probes in liposomes / Paul S. Uster ... [et al.] -- Permeabilizing mammalian cells to macromolecules / Gary A. Weisman ... [et al.] |
|
Transport machinery: An overview / George Sachs ... [et al.] -- Survey of carrier methodology: Strategy for identification, isolation, and characterization of transport systems / Martin Klingenberg, Sidney Fleischer and Becca Fleischer -- Kinetics of transport: Analyzing, testing, and characterizing models using kinetic approaches / Wilfred D. Stein, Sidney Fleischer and Becca Fleischer -- Kinetics of ion movement mediated by carriers and channels / Olaf Sparre Andersen, Sidney Fleischer and Becca Fleischer -- Inhibition kinetics of carrier systems / Rosa Devés ... [et al.] -- Design of simple devices to measure solute fluxes and binding in monolayer cell cultures / Jaydutt V. Vadgama, Sidney Fleischer and Becca Fleischer -- Utilization of binding energy and coupling rules for active transport and other coupled vectorial processes / William P. Jencks, Sidney Fleischer and Becca Fleischer -- Generation of steady-state rate equations for enzyme and carrier-transport mechanisms: A microcomputer program / Keith R. Runyan ... [et al.] -- Liposome preparation and size characterization / Martin C. Woodle ... [et al.] -- Preparation of microcapsules from human erythrocytes: Use in transport experiments of glutathione and its S-conjugate / Takahito Kondo, Sidney Fleischer and Becca Fleischer -- Planar lipid-protein membranes: Strategies of formation and of detecting dependencies of ion transport functions on membrane conditions / Hansgeorg Schindler, Sidney Fleischer and Becca Fleischer -- Spontaneous insertion of integral membrane proteins into preformed unilamellar vesicles / David Zakim ... [et al.] |
| Summary |
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Biological transport.
|
|
Cell separation.
|
|
Epithelial cells.
|
|
Membranes (Biology)
|
|
Biological Transport.
|
|
Cell Membrane Permeability.
|
|
Cell Separation.
|
|
Epithelium.
|
| Form |
Electronic book
|
| Author |
Fleischer, Becca.
|
|
Fleischer, Sidney.
|
| ISBN |
0121820726 (electronic bk.) |
|
9780121820725 (electronic bk.) |
|
