| Description |
1 online resource (xi, 311 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; volume 1850 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1850.
|
| Contents |
Transient gene expression in suspension HEK293-EBNA1 cells / Denis L'Abbe, Louis Bisson, Christian Gervais, Eric Grazzini, and Yves Durocher -- Transient expression of recombinant membrane-eGFP fusion proteins in HEK293 cells / Joanna Pieprzyk, Samuel Pazicky, and Christian Low -- PEI-mediated transient gene expression in CHO cells / Yashas Rajendra -- Stable expression by lentiviral transduction of cells / Natascha Godecke, Hansjorg Hauser, and Dagmar Wirth -- Inducible protein production in 293 cells using the piggyBac Transposon System / Iacovos P. Michael and Andras Nagy -- Recombinant CHO cell pool generation using piggyBac transposon system / Sowmya Balasubramanian -- Genome engineering of hybridomas to generate stable cell lines for antibody expression / Cristina Parola, Derek M. Mason, Andreas Zingg, Daniel Neumeier, and Sai T. Reddy -- Protein expression via transient transfection of mammalian cells in a WAVE bioreactor / David I. Fisher -- CHO and HEK293 cultivation and transfection in single-use orbitally shaken bioreactors / David L. Hacker, Laurence Durrer, and Soraya Quinche -- Bench-scale stirred-tank bioreactor for recombinant protein production in chinese hamster ovary (CHO) cells in suspension / Dominique T. Monteil and Jeffrey Kuan -- Continuous and integrated expression and purification of recombinant antibodies / Sebastian Vogg, Moritz Kai Florian Wolf, and Massimo Morbidelli -- High throughput transfection of HEK293 cells for transient protein production / Tia A. Arena, Peter D. Harms, and Athena W. Wong -- Microfluidic transfection for high-throughput mammalian protein expression / Kristina Woodruff and Sebastian J. Maerkl -- Genome-wide high-throughput RNAi screening for identification of genes involved in protein production / Sarah Inwood, Michael J. Betenbaugh, Madhu Lal, and Joseph Shiloach -- Targeting miRNAs with CRISPR/Cas9 to improve recombinant protein production of CHO cells / Kevin Kellner, Ankur Solanki, Thomas Amann, Nga Lao, and Niall Barron -- Application of the CRISPR/Cas9 gene editing method for modulating antibody fucosylation in CHO cells / Qiong Wang, Cheng-Yu Chung, Julian N. Rosenberg, Geng Yu, and Michael J. Betenbaugh -- Scalable production and purification of adeno-associated viral vectors (AAV) / Daniel Blessing, Nicole Deglon, and Bernard L. Schneider -- Considerations in the use of codon optimization for recombinant protein expression / Vincent P. Mauro and Stephen A. Chappell -- Versatile cell-free protein synthesis systems based on chinese hamster ovary cells / Lena Thoring and Stefan Kubick |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed September 21, 2018) |
| Subject |
Recombinant proteins -- Laboratory manuals
|
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Gene expression -- Laboratory manuals
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Recombinant proteins.
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Recombinant Proteins
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Proteins.
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Science -- Life Sciences -- Biochemistry.
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Gene expression
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Recombinant proteins
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| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Hacker, David L., editor
|
| ISBN |
9781493987306 |
|
1493987305 |
|
