| Description |
1 online resource (viii, 179 pages) : illustrations |
| Series |
Springer protocols handbooks, 1949-2448 |
|
Springer protocols (Series) 1949-2448
|
| Contents |
Animal coronaviruses : a brief introduction / Leyi Wang and Yan Zhang -- Immunohistochemical staining for detection of porcine epidemic diarrhea virus in tissues / Kwonil Jung -- Virus neutralization assay for turkey coronavirus infection / Yi-Ning Chen, Ching Ching Wu, and Tsang Long Lin -- Recombinant turkey coronavirus nucleocapsid protein expressed in Escherichia coli / Chien Chang Loa, Ching Ching Wu, and Tsang Long Lin -- Antibody-capture enzyme-linked immunosorbent assay for detection of antibody to turkey coronavirus using infectious bronchitis virus or recombinant nucleocapsid protein as coating antigen / Chien Chang Loa [and others] -- Reverse genetics of avian coronavirus infectious bronchitis virus / Sarah M. Keep, Erica Bickerton, and Paul Britton -- Vaccinia virus-based reverse genetics for feline coronaviruses / Gergely Tekes -- Real-time RT-PCR detection of equine coronavirus / Fabien Miszczak [and others] -- Detection of bovine coronavirus by conventional reverse transcription polymerase chain reaction / Amauri Alcindo Alfieri, Alice Fernandes Alfieri, and Elisabete Takiuchi -- Real-time reverse transcription polymerase chain reaction for rapid detection of transmissible gastroenteritis virus / Ramesh Vemulapalli -- RT-PCR assay for detection of infectious bronchitis coronavirus serotypes / Junfeng Sun and Shengwang Liu -- Multiplex polymerase chain reaction for differential detection of turkey coronavirus from chicken infectious bronchitis virus and bovine coronavirus / Chien Chang Loa, Ching Ching Wu, and Tsang Long Lin -- Real-time reverse transcription-polymerase chain reaction for detection and quantitation of turkey coronavirus RNA in feces and intestine tissues / Yi-Ning Chen, Ching Ching Wu, and Tsang Long Lin -- PCR amplification and sequencing analysis of full-length turkey coronavirus spike gene / Yi-Ning Chen [and others] -- Feline coronavirus RT-PCR assays for feline infectious peritonitis diagnosis / Takehisa Soma -- Genotype-specific detection of ferret coronavirus by conventional and real-time reverse transcription polymerase chain reaction / Annabel G. Wise, Matti Kiupel, and Roger K. Maes -- Molecular characterization of canine coronavirus / Rita de Cássia Nasser Cubel Garcia -- Whole-genome sequencing of porcine epidemic diarrhea virus by illumina MiSeq platform / Leyi Wang [and others] -- Next-generation sequencing for porcine coronaviruses / Douglas Marthaler [and others] |
| Summary |
Annotation This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Coronaviruses.
|
|
Coronaviridae
|
|
Coronaviruses
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Wang, Leyi, editor
|
| ISBN |
9781493934140 |
|
1493934147 |
|
