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Title Chemical genomics : reviews and protocols / edited by Edward D. Zanders
Published Totowa, N.J. : Humana Press, [2005]


Description 1 online resource (xi, 279 pages) : illustrations
Series Methods in molecular biology ; v. 310
Methods in molecular biology (Clifton, N.J.) ; v. 310
Contents Introduction to chemical genomics / Paul R. Caron -- Chemistry for chemical genomics / Lutz Weber -- Computer-aided design of small molecules for chemical genomics / Philip M. Dean -- Design, synthesis, and screening of biomimetic ligands for affinity chromatography / Ana Cecília A. Roque, Geeta Gupta, and Christopher R. Lowe -- The role and application of in silico docking in chemical genomics research / Aldo Jongejan [and others] -- Synthesis of complex carbohydrates and glyconjugates: enzymatic synthesis of globotetraose using 1,3-N-acetylgalactosaminyltransferase LgtD from Haemophilus infuenzae strain Rd / Kang Ryu [and others] -- High-throughput cloning for proteomics research / Sharon A. Doyle
Screening for the expression of soluble recombinant protein in Escherichia coli / Sharon A. Doyle -- High-throughput purification of hexahistidine-tagged proteins expressed in Escherichia coli / Michael B. Murphy and Sharon A. Doyle -- The wheat germ cell-free expression system: methods for high-throughput materialization of genetic information / Tatsuya Sawasaki [and others] -- Advances in genome-wide protein expression using the wheat germ cell-free system / Yaeta Endo and Tatsuya Sawasaki -- Production of proteins for NMR studies using the wheat germ cell-free system / Toshiyuki Kohno -- Adenoviral expression of reporter proteins for high-throughput cell-based screening / Jon Hoyt and Randall W. King
Summary Chemical genomics is an exciting new field that promises to transform biological chemistry into a high-throughput industrialized process, much in the same way that genomics has transformed molecular biology. In Chemical Genomics: Reviews and Protocols, leading edge researchers introduce the uninitiated into the various "flavors" of chemical genomics and describe a series of readily reproducible techniques for applications in organic chemistry, protein chemistry, and computer science, with emphasis on the high-throughput approaches that are the essence of chemical genomics. The techniques range from the classic design of small molecules as affinity ligands for specific protein families, to in silico docking for computational approaches, to protein-small molecule interactions, to producing highly diverse collections of proteins, carbohydrates, and small molecules for use in arrays containing large numbers of these molecules. There are also methods for the production of proteins and carbohydrates using different expression systems, for analyzing proteins using mass spectrometry, and for arraying proteins and carbohydrates on solid supports. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Chemical Genomics: Reviews and Protocols offers researchers an accessible introduction by pioneers to the key areas and methods of chemical genomics, as well as its use in the discovery and evaluation of novel drug targets
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Genomics.
Biochemical genetics.
DNA microarrays.
Combinatorial chemistry.
Pharmaceutical technology.
Genetics -- Technique.
Life sciences.
Physical sciences.
Molecular probes.
Genomics -- methods -- Laboratory Manuals
Combinatorial Chemistry Techniques -- Laboratory Manuals
Ligands -- Laboratory Manuals
Proteins -- chemical synthesis -- Laboratory Manuals
Microarray Analysis
Investigative Techniques
Molecular Probe Techniques
Laboratory Chemicals
Publication Formats
Amino Acids, Peptides, and Proteins
Technology, Pharmaceutical
Computational Biology
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Specialty Uses of Chemicals
Genetic Techniques
Microchip Analytical Procedures
Publication Characteristics
Chemicals and Drugs
Chemical Actions and Uses
Chemistry Techniques, Analytical
Biological Science Disciplines
Natural Science Disciplines
Disciplines and Occupations
Protein Array Analysis
Combinatorial Chemistry Techniques
Molecular Probes
Laboratory Manuals
Protein Array Analysis -- methods -- Laboratory Manuals
Molecular Biology
biological sciences.
physical sciences.
SCIENCE -- Life Sciences -- Genetics & Genomics.
Physical sciences
Pharmaceutical technology
Molecular probes
Life sciences
Genetics -- Technique
Biochemical genetics
Combinatorial chemistry
DNA microarrays
Health & Biological Sciences.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Zanders, Edward D.
LC no. 2005013118
ISBN 9781592599486