| Description |
1 online resource (xv, 489 pages) : illustrations |
| Series |
Methods in molecular biology ; 322 |
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Methods in molecular biology (Clifton, N.J.) ; v. 322
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| Contents |
Resources for genetic and genomic studies of xenopus -- The physiology of the xenopus laevis ovary -- Oocyte isolation and enucleation -- Xenopus tropicalis oocytes: more than just a beautiful genome -- Oocyte expression with injection of purified T7 RNA polymerase -- Visualization of the cytoskeleton in xenopus ooxytes and eggs by confocal immunofluorescence microscopy -- Multiphoton laser scanning microscopy as a tool for Xenopus oocyte research -- Imaging Ca² signals in xenopus oocytes -- Chromosomal DNA replication in a soluble cell-free system derived from xenopus eggs -- Chromatin assembly of DNA templates microinjected into xenopus oocytes -- Pre-mRNA splicing in the nuclei of xenopus oocytes -- Chromatin immunoprecipitation for studying transcriptional regulation in xenopus oocytes and tadpoles -- Cytoplasmic mRNA polyadenylation and translation assays |
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Xenopus egg extracts: a model system to study proprotein convertases -- Analysis of molecular chaperones using a xenopus oocyte protein refolding assay -- Ubiquitin-mediated protein degradation in xenopus egg extracts -- Introduction to nucleocytoplasmic transport: molecules and mechanisms -- Use of xenopus laevis oocyte nuclei and nuclear envelopes in nucleocytoplasmic transport studies -- Nuclear pore complex structure and plasticity revealed by electron and atomic force microscopy -- In vitro study of nuclear assembly and nuclear import using xenopus egg extracts -- Use of intact xenopus oocytes in nculeocytoplasmic transport studies -- Studying the mechanosensitivity of voltage-gated channels using oocyte patches -- Oocytes as an expression system for studying receptor/channel targets of drugs and pesticides -- Microtransplantation of neurotransmitter receptors from cells to xenopus oocyte membranes: new procedure for ion channel studies |
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Reconstitution of golgi disassembly by mitotic xenopus egg extracts in semi-intact MDCK cells -- Exploring RNA virus replication in xenopus oocytes -- Study of apoptosis in vitro using the xenopus egg extract reconstitution system -- Studying fertilization in cell-free extracts: focusing on membrane/lipid raft functions and proteomics -- Localized sampling, electrophoresis, and biosensor analysis of xenopus laevis cytoplasm for subcellular biochemical assays -- Monitoring protein kinase A activities using expressed substrate in live cells -- Using xenopus oocyte extracts to study signal transduction -- Oocyte extracts for the study of meiotic M-M transition -- Methods for studying spindle assembly and chromosome condensation in xenopus egg extracts |
| Summary |
Although Xenopus oocytes are very hardy and robust in terms of experimentation and offer unusually simple and inexpensive opportunities for the delivery of mRNA to the cytoplasm and DNA to the nucleus, they are underutilized as an experimental model. In Xenopus Protocols: Cell Biology and Signal Transduction, hands-on researchers describe standard and cutting-edge techniques for using Xenopus oocytes and oocyte/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance (for example, 50-70 ng catalytic subunit of PKA per oocyte), its striking protein translation capacity (200-400 ng protein/oocyte per day), and its breathtaking possibilities for the assembly of infectious viral particles by single-cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction and cover all the major uses of oocytes/extracts as experimental models. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Xenopus Protocols: Cell Biology and Signal Transduction serves perfectly to instruct novices in the use of Xenopus oocytes/extracts, as well as seasoned investigators interested in developing novel models and uses |
| Bibliography |
Includes bibliographical references |
| Notes |
English |
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Print version record |
| Subject |
Xenopus laevis -- Eggs -- Laboratory manuals
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Xenopus laevis -- Cytology -- Laboratory manuals
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Cellular signal transduction -- Laboratory manuals
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Cellular signal transduction.
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Oocytes -- cytology
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Signal Transduction
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Xenopus -- embryology
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SCIENCE -- Life Sciences -- Cell Biology.
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Cellular signal transduction
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Xenopus laevis -- Cytology
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Xenopus laevis -- Eggs
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| Genre/Form |
Handbooks and manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Liu, X. Johné.
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| ISBN |
1588293629 |
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9781588293626 |
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9781597450003 |
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1597450006 |
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1280837276 |
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9781280837272 |
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9786610837274 |
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6610837279 |
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