Title Baculovirus and insect cell expression protocols / edited by David W. Murhammer

Edition Third edition Published New York, NY : Humana Press, 2016

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Description 1 online resource (xii, 508 pages) : illustrations (some color) Series Methods in Molecular Biology, 1064-3745 ; 1350 Methods in molecular biology (Clifton, N.J.) ; v. 1350. 1064-3745 Contents Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior -- Introduction to Baculovirus Molecular Biology -- Baculovirus Transfer Vectors -- Recombinant Baculovirus Isolation -- Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System -- Available Lepidopteran Insect Cell Lines -- Lepidopteran Insect Cell Line Isolation from Insect Tissue -- Development Of Serum-Free Media For Lepidopteran Insect Cell Lines -- Routine Maintenance And Storage Of Lepidopteran Insect Cell -- Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System -- Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System -- Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors -- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae -- Production Of Virus-Like Particles For Vaccination -- Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells -- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression -- Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression -- Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression -- Introduction To The Use Of Baculoviruses As Biological Insecticides -- Baculovirus Insecticide Production In Insect Larvae -- Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses -- Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants -- Tubular Bioreactor for Probing Baculovirus Infection and Protein Production -- Gene Silencing In Insect Cells Using Rnai -- Using the Baculovirus/Insect Cell System to Study Apoptosis -- Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells Summary Annotation With new and up-to-date methods and protocols, this volume discusses, among other topics, the step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism; methods to isolate new cell lines and develop serum-free mediums; routine maintenance and storage of insect cell lines and baculoviruses; and small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture, and in insect larvae Notes Includes index Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed February 4, 2016) Subject Baculoviruses -- Genetics -- Laboratory manuals Genetic engineering -- Laboratory manuals Virus-vector relationships -- Laboratory manuals Insect cell biotechnology -- Laboratory manuals Gene expression. Genetic vectors. Genetics -- Technique. Baculoviridae -- genetics Gene Expression Genetic Vectors Genetic Techniques Genetics -- Technique Genetic vectors Gene expression Baculoviruses -- Genetics Genetic engineering Insect cell biotechnology Virus-vector relationships Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Murhammer, David W., editor ISBN 9781493930432 1493930435

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