Title Antiviral resistance in plants : methods and protocols / edited by John M. Watson and Ming-Bo Wang

Published New York : Humana Press : Springer, ©2012

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Description 1 online resource (xi, 380 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; 894 Methods in molecular biology (Clifton, N.J.) ; v. 894. 1064-3745 Contents Historical overview of RNAi in plants / John A. Lindbo -- RNA silencing and antiviral defense in plants / Claire Agius, Andrew L. Eamens, Anthony A. Millar, John M. Watson, and Ming-Bo Wang -- Biological chemistry of virus-encoded suppressors of RNA silencing : an overview / Rustem T. Omarov and Herman B. Scholthof -- Virus-induced gene silencing using begomovirus satellite molecules / Xueping Zhou and Changjun Huang -- Strategies for viral cross protection in plants / Changyong Zhou and Yan Zhou -- Tobacco rattle virus (TRV)-based virus-induced gene Silencing / Shawn Bachan and Savithramma P. Dinesh-Kumar -- Virus-induced gene silencing in hexaploid wheat using barley stripe mosaic virus vectors / Steven R. Scofield and Amanda S. Brandt -- Characterization of plant virus-encoded gene silencing suppressors / Hajime Yaegashi, Masamichi Isogai, and Nobuyuki Yoshikawa -- Generation of plant small RNA cDNA libraries for high-throughput sequencing / Qian-Hao Zhu and Christopher A. Helliwell -- Modeling antiviral resistance in plants / Robert S. Anderssen and Peter M. Waterhouse -- Isolation and detection of small RNAs from plant tissues / Neil A. Smith and Andrew L. Eamens -- Isolation and analysis of small RNAs from virus-infected plants / Shaun J. Curtin, Agustín Zsögön, John M. Watson, and Peter M. Waterhouse -- Use of hairpin RNA constructs for engineering plant virus resistance / Neena Mitter and Ralf G. Dietzgen -- Artificial microRNAs for plant virus resistance / Jing Qu, Jian Ye, and Rongxiang Fang -- Isolation and biochemical analysis of plant small RNAs / H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau -- Isolation and analysis of plant RNA-dependent RNA polymerases / Julien Curaba -- Plant viroids : isolation, characterization/detection, and analysis / Robert A. Owens, Teruo Sano, and Nuria Duran-Vila -- Structural and functional analysis of CMV satellite RNAs in RNA silencing / Hanako Shimura and Chikara Masuta -- Virus-Induced Gene Silencing in Soybean / Aardra Kachroo and Said Ghabrial -- Chimeric, infectious, and stable virus transcripts to study RNA silencing in "Dark Green" Islands / Robin M. MacDiarmid -- Rolling circle amplification-mediated long hairpin RNA library construction in plants / Lei Wang and Yun-Liu Fan -- Interaction between plant viroid-induced symptoms and RNA silencing / Vicente Pallas, German Martinez, and Gustavo Gomez -- Identification of small molecule inhibitors of tomato bushy stunt virus replication / Zsuzsanna Sasvari and Peter D. Nagy -- Expression of dominant-negative mutants to study host factors affecting plant virus infections / Daniel Barajas and Peter D. Nagy Summary Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait Analysis Life sciences Medical virology Botany Plant diseases Plant Sciences Plant Pathology Virology planten plants plantenvirussen plant viruses ziekteresistentie disease resistance plant-microbe interactions laboratoriummethoden laboratory methods protocollen protocols antivirale planten antiviral plants uitschakelen van genexpressie gene silencing Plant Viruses Microbe-Plant Relations Plantenvirussen Microbe-plant relaties Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (Springer Protocols, viewed Oct. 17, 2012) Subject Plants -- Virus resistance -- Laboratory manuals Plant immunology -- Laboratory manuals Viruses. Plant physiology. Antisense RNA. Autoimmunity. Immunity. Non-coding RNA. RNA. Nucleic acids. Genetic regulation. Immune system. Small interfering RNA. RNA interference. Plant viruses. Publication Formats Viruses RNA, Small Untranslated Adaptive Immunity Plant Physiological Phenomena Gene Silencing RNA, Antisense Autoimmunity Antisense Elements (Genetics) Immunity Phenomena and Processes Organisms RNA, Untranslated RNA Epigenesis, Genetic Publication Characteristics Nucleic Acids Gene Expression Regulation Immune System Phenomena Nucleic Acids, Nucleotides, and Nucleosides Genetic Phenomena Chemicals and Drugs RNA, Small Interfering Plant Immunity Laboratory Manuals RNA Interference Plant Viruses Immune System Viruses Small interfering RNA RNA interference RNA Plant viruses Plant physiology Nucleic acids Non-coding RNA Immunity Immune system Genetic regulation Autoimmunity Antisense RNA Plant immunology Plants -- Virus resistance Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Watson, J. M. (John Matheson) Wang, Ming-Bo, 1962- ISBN 9781617798825 1617798827 1617798819 9781617798818

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