Host restriction of HIV-1 by APOBEC3 and viral evasion through Vif -- Interactions of viral protein U (Vpu) with cellular factors -- TRIM5alpha -- Human immunodeficiency virus type-1 Gag and host vesicular trafficking pathways -- The roles of tetraspanins in HIV-1 replication -- Imaging of HIV/host protein interactions -- Virological and cellular roles of the transcriptional coactivator LEDGF/p75 -- Implications of Nef: host cell interactions in viral persistence and progression to AIDS -- Vpr and its interactions with cellular proteins
Summary
The authors seek to understand the assembly process of human immunodeficiency virus (HIV) and to develop a vaccine that can effectively neutralize HIV. HIV assembly is a process directed by the viral Gag polyprotein. Gag is a myristoylated precursor protein that is translated in the cytoplasm and then traffics to the plasma membrane or to endosomal vesicles for assembly. The authors have recently described an interaction between Gag and the delta subunit of the AP-3 adaptor protein complex (1). The mechanism and structural basis for this interaction is now under intense study. Our hypothesis i
