Title Virus culture : a practical approach / edited by Alan J. Cann

Published Oxford ; New York : Oxford University Press, ©1999

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Description 1 online resource (xviii, 272 pages) : illustrations Series Practical approach series ; 208 Practical approach series ; 208. Contents Intro; Contents; List of Contributors; Abbreviations; 1. Cell culture; 1. Introduction; Sources of cell cultures; Suppliers of cell cultures; Types of cell culture; 2. Culture media; Basal media; Serum; Serum-free media; Antibiotics; 3. Good practice in cell culture; 4. Principles of cell banking; 5. Subculture of cells; 6. Quantification of cell cultures; 7. Cryopreservation and storage of cell lines; Storage of cryopreserved cell stocks; Resuscitation of cryopreserved cell stocks; 8. Quality control testing of cell lines; Preparation of cell cultures prior to testing; Mycoplasma Bacteria and fungiViruses; Isoenzyme analysis; Karyotyping; DNA fingerprinting; 9. Transportation; Shipment of growing cultures; Shipment of frozen materials on dry ice (solid carbon dioxide); References; 2. Virus isolation; 1. Why culture viruses?; 2. Viruses not amenable to cultivation; 3. Treatment of clinical specimens for virus isolation; 4. Identification of virus replication; 5. Passaging of viruses; 6. Confirmation of virus isolation; 7. Adaptations of cell culture; Haemadsorption test; Detection of early antigen fluorescent foci (DEAFF test); 8. Conclusions; References 3. Concentration and purification of viruses1. The need for virus concentration and purification; Practical considerations; 2. Virus concentration: general principles; 3. Purification of virus: identification of virus-containing fractions; 4. Purification by ultracentrifugation; Types of ultracentrifugation gradient; Forming gradients; 5. Assessment of purity; References; 4. Assays for virus infection; 1. Introduction; 2. Identification of viruses isolated in cell culture; Cytopathic effects; Virus infectivity assays; Immunofluorescence; Electron microscopy Other methods of identifying viruses growing in cell cultureDetection of virus pre-cytopathic effects; Detection of viruses not producing a cytopathic effect; 3. Virus antigen detection; Immunofluorescence; Particle agglutination; Antigen-capture ELISA; 4. Virus genome detection; Nucleic acid extraction; Genome detection assays; Quantitative nucleic acid amplification techniques; 5. Detection of specific anti-viral antibodies; Virus-specific IgM detection; Detection of virus-specific IgG; References; 5. Electron microscopy of viruses; 1. Introduction 2. Virus structure and strategies for virus detection3. Specimen preparation; Centrifugation; Ultracentrifugation; Ultrafiltration; Precipitation; Bioaffinity techniques; 4. EM grids and support films; 5. Negative staining; Negative stains: properties; Negative staining procedures; Quantitative aspects: measuring and counting in TEM; 6. Embedding and thin sectioning; Fixation and staining; Embedding; Thin sectioning; Post-staining; 7. Immuno-electron microscopy (IEM); Antibodies: properties and usage; Immuno-negative staining; Immuno-labelling of thin sections Summary Viruses have properties that are distinct from other living organisms and so require different methods to culture them. This volume provides a broad treatment of the principles and practice of virus culture and will be of interest to all those involved in virus culture including academic, industrial, and clinical research groups Bibliography Includes bibliographical references and index Notes English Print version record Subject Virology -- Cultures and culture media. Monoclonal antibodies. Viral vaccines. Virology -- methods Virus Cultivation Antibodies, Monoclonal Antiviral Agents -- pharmacology Microscopy, Electron -- methods Plant Viruses -- isolation & purification Viral Vaccines Virus Cultivation -- methods SCIENCE -- Life Sciences -- Biology. SCIENCE -- Life Sciences -- Microbiology. Viral vaccines Monoclonal antibodies Virology -- Cultures and culture media Zellkultur Methode Viren Genre/Form Electronic books Form Electronic book Author Cann, Alan ISBN 058548385X 9780585483856 9780191565847 0191565849 1280375620 9781280375620 9786610375622 6610375623

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