| Description |
1 online resource (xiv, 269 pages) : color illustrations |
| Series |
Methods in molecular biology ; 1532 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1532
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| Contents |
Current trends and alternative scenarios in EBV research / Janos Minarovits and Hans Helmut Niller -- Epstein-Barr virus : clinical ciagnostics / Hans-Helmut Niller and Georg Bauer -- Establishment of EBV-infected lymphoblastoid cell lines / Noemi Nagy -- Generation and infection of organotypic cultures with Epstein-Barr virus / Rachel M. Temple, Craig Meyers, and Clare E. Sample -- Affinity purification-mass spectroscopy methods for identifying Epstein-Barr virus-host interactions / Anna A. Georges and Lori Frappier -- Use of 3D telomere FISH for the characterization of the nuclear architecture in EBV-positive hodgkin's lymphoma / Hans Knecht and Sabine Mai -- Analysis of EBV transcription using high-throughput RNA sequencing / Tina O'Grady, Melody Baddoo, and Erik K. Flemington -- Analysis of viral promoter usage in EBV-infected cell lines : a comparison of qPCR following conventional RNA isolation and nuclear run-on assay / Kalman Szenthe and Ferenc Banati -- Analysis of viral and cellular microRNAs in EBV-infected cells / Rebecca L. Skalsky -- Isolation and characterization of exosomes released by EBV-immortalized cells / Gulfaraz Khan and Waqar Ahmed -- Functional analysis of exosomes derived from EBV-infected cells / Gulfaraz Khan and Pretty S. Philip -- Terminal repeat analysis of EBV genomes / Ferenc Bánáti, Anita Koroknai, and Kálmán Szenthe -- Characterization of EBV promoters and coding regions by sequencing PCR-amplified DNA fragments / Kalman Szenthe and Ferenc Bánáti -- Use of chromatin precipitation coupled to DNA sequencing (ChIP-Seq) for the analysis of Zta binding to the human and EBV genome / Anja Godfrey, Sharada Ramasubramanyan, and Alison J. Sinclair -- Analysis of viral epigenotypes using bisulfite sequencing : a detailed protocol for the crucial bisulfite modification and PCR amplification steps / Daniel Salamon -- Analysis of viral epigenotypes using chromatin immunoprecipitation / Ferenc Bánáti and Kálmán Szenthe -- Mice with reconstituted human immune system components as a tool to study immune cell interactions in EBV infection / Frank Heuts and Noemi Nagy -- Generation and analysis of humanized mouse model of EBV infection / Ken-Ichi Imadome and Shigeyoshi Fujiwara -- EBV-directed T cell therapeutics for EBV-associated lymphomas / Lauren P. McLaughlin, Stephen Gottschalk, Cliona M. Rooney, and Catherine M. Bollard |
| Summary |
This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the analysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Epstein Barr-Virus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocols, viewed December 6, 2016) |
| Subject |
Epstein-Barr virus -- Laboratory manuals
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Human beings.
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Humans
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Herpesvirus 4, Human -- physiology
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Herpesvirus 4, Human -- isolation & purification
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Immunoassay -- methods
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Viral Proteins -- chemistry
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Viral Proteins -- genetics
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Virus Cultivation -- methods
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Homo sapiens (species)
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Epstein-Barr virus
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| Form |
Electronic book
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Author |
Minarovits, Janos, editor
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Niller, Hans Helmut, editor
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| ISBN |
9781493966554 |
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1493966553 |
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1493966537 |
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9781493966530 |
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