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Book Cover
Author Yuan, Sarah Y

Title Regulation of endothelial barrier function / Sarah Y. Yuan, Robert R. Rigor
Published San Rafael [Calif.] : Morgan & Claypool Life Sciences, ©2011


Description 1 online resource (PDF file (xi, 146 pages : illustrations))
Series Integrated systems physiology, from molecule to function to disease ; #12
Colloquium series on integrated systems physiology ; #12.
Contents 880-01 1. Introduction -- 2. Structure and function of exchange microvessels -- Microvascular blood-tissue exchange -- Physical forces governing fluid filtration -- Solute transport across the microvascular wall -- Convection vs. diffusion -- The capillary pore theory -- Physiological factors affecting fluid/solute flux -- Hemodynamics -- Lymphatic drainage -- Endothelial barrier properties -- Leukocytes and endothelial barriers -- 3. Methods for measuring permeability -- Analysis of fluid filtration (Kf) -- Measuring Kf in intact tissues -- Analysis of hydraulic conductivity (Lp) -- Measuring Lp with colorimetric dyes -- Analysis of solute permeability coefficient (Ps) -- The isolated, perfused microvessel technique -- Analysis of the osmotic reflection coefficient ([sigma]) -- General indicators of plasma extravasation -- Intravital microscopic measurement of transvascular flux -- Assessment of barrier function in cultured endothelial cells -- Transwell solute flux assays -- Transendothelial electrical resistance measurements -- 4. The endothelial barrier -- Transcellular permeability: vesicular transcytosis -- Paracellular permeability: cell-cell junctions -- Adherens junctions -- Tight junctions -- Extracellular barriers: focal adhesions -- The endothelial cytoskeleton -- Microtubules -- Intermediate filaments -- Actin filaments -- Actomyosin contractile machinery -- 5. Signaling mechanisms in the regulation of endothelial permeability -- Initiation of signaling: endothelial receptors -- Receptor tyrosine kinases -- G-protein-coupled receptors -- Integrin receptors -- Intracellular signal transduction: second messengers -- Calcium -- Camp -- No-CGMP -- Protein kinases and phosphatases -- Myosin light-chain kinase -- Myosin light-chain phosphatase -- Src family kinases -- Protein kinase C -- Focal adhesion kinase -- Small GTPases -- Proteases and the extracellular matrix -- MMPs -- ADAMs -- Fibrinogen -- 6. Endothelial barrier protectors -- Phospholipids -- Sphingosine-1-phosphate -- Activated protein C -- Angiopoietins -- 7. Pathophysiology and clinical relevance -- Burn edema -- ALI/ARDS and sepsis -- Ischemia-reperfusion injury -- Diabetes mellitus -- Tumor angiogenesis and metastatic migration
880-01/(S chapter 3: Methods for Measuring PermeabilityAnalysis of Fluid Filtration (Kf); Measuring Kf in Intact Tissues; Analysis of Hydraulic Conductivity (Lp); Measuring Lp With Colorimetric Dyes; Analysis of Solute Permeability Coefficient (Ps); The Isolated, Perfused Microvessel Technique; Analysis of the Osmotic Reflection Coefficient (σ); General Indicators of Plasma Extravasation; Intravital Microscopic Measurement of Transvascular Flux; Assessment of Barrier Function in Cultured Endothelial Cells; Transwell Solute Flux Assays; Transendothelial Electrical Resistance Measurements
Summary The vascular endothelium lining the inner surface of blood vessels serves as the first interface for circulating blood components to interact with cells of the vascular wall and surrounding extravascular tissues. In addition to regulating blood delivery and perfusion, a major function of vascular endothelia, especially those in exchange microvessels (capillaries and postcapillary venules), is to provide a semipermeable barrier that controls blood-tissue exchange of fluids, nutrients, and metabolic wastes while preventing pathogens or harmful materials in the circulation from entering into tissues. During host defense against infection or tissue injury, endothelial barrier dysfunction occurs as a consequence as well as cause of inflammatory responses. Plasma leakage disturbs fluid homeostasis and impairs tissue oxygenation, a pathophysiological process contributing to multiple organ dysfunction associated with trauma, infection, metabolic disorder, and other forms of disease. In this book, we provide an updated overview of microvascular endothelial barrier structure and function in health and disease. The discussion is initiated with the basic physiological principles of fluid and solute transport across microvascular endothelium, followed by detailed information on endothelial cell-cell and cell-matrix interactions and the experimental techniques that are employed to measure endothelial permeability. Further discussion focuses on the signaling and molecular mechanisms of endothelial barrier responses to various stimulations or drugs, as well as their relevance to several common clinical conditions. Taken together, this book provides a comprehensive analysis of microvascular endothelial cell and molecular pathophysiology. Such information will assist scientists and clinicians in advanced basic and clinical research for improved health care
Analysis Microvascular permeability
Endothelial barrier
Cell-cell junction
Cell-matrix adhesion
Signal transduction
Bibliography Includes bibliographical references
Notes English
Subject Vascular endothelium.
Endothelium, Vascular
MEDICAL -- Physiology.
SCIENCE -- Life Sciences -- Human Anatomy & Physiology.
Vascular endothelium
Form Electronic book
Author Rigor, Robert R. (Robert Rusk)
ISBN 9781615041206
Other Titles Colloquium digital library of life sciences: Collection 1
NCBI Bookshelf