| Description |
1 online resource (xiii, 411 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1034 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1034. 1064-3745
|
| Contents |
Alloreactivity / Sidonia B.G. Eckle, Jamie Rossjohn, and James McCluskey -- Antibodies in Transplantation : The Effects of HLA and Non- HLA Antibody Binding and Mechanisms of Injury / Nicole M. Valenzuela and Elaine F. Reed -- Cell Mediated Rejection / Kathryn J. Wood, Masaaki Zaitsu, and Ryoichi Goto -- Transplantation Tolerance / Colin Brinkman [and others] -- Composite Tissue Transplantation / Gerald Brandacher -- Transplantation of the Sensitized Patient: Histocompatibility Testing / Robert A. Montgomery, Mary S. Leffell, and Andrea A. Zachary -- Thoracic Organ Transplantation : Laboratory Methods / Jignesh K. Patel and Jon A. Kobashigawa -- HLA Typing by Sequence-Specific Primers / Mike Bunce and Ben Passey -- Human Leukocyte Antigen (HLA) Typing by DNA Sequencing / Ana Lazaro [and others] -- Next-Generation HLA Sequencing Using the 454 GS FLX System / Elizabeth A. Trachtenberg and Cherie L. Holcomb -- Chimerism Testing by Quantitative PCR Using Indel Markers / Ketevan Gendzekhadze, Laima Gaidulis, and David Senitzer -- Gene-Specific PCR Typing of Killer Cell Immunoglobulin- Like Receptors / Raja Rajalingam and Elham Ashouri -- Complement-Dependent Cytotoxicity Crossmatch / Jeremy Ryan Pena, Donna Fitzpatrick, and Susan L. Saidman -- Lymphocyte Crossmatching by Flow Cytometry / Robert A. Bray -- Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA-Specific Antibodies / John D. Smith and Marlene L. Rose -- C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens / Ge Chen and Dolly B. Tyan -- Tetramer Staining for the Detection of HLA-Specific B cells / Donna P. Lucas, Mary S. Leffell, and Andrea A. Zachary -- Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood / Annette M. Jackson, Donna P. Lucas, and Jessica L. Badders -- Detection of Antibodies to the Angiotensin II-Type 1 Receptor in Transplantation / Duska Dragun -- Detection of Antibodies to Self-Antigens (K-alpha 1 Tubulin, Collagen I, II, IV, and V, Myosin, and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA) / Nayan J. Sarma, Venkataswarup Tiriveedhi, and T. Mohanakumar -- Cylex ImmuKnow Cell Function Assay / Adriana Zeevi and John Lunz -- Detection of Intracellular Cytokines / Nancy L. Reinsmoen and Chih-Hung Lai -- Tolerogenic Dendritic Cells and Induction of T Suppressor Cells in Transplant Recipients / George Vlad and Nicole Suciu-Foca -- Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA / Tara K. Sigdel and Minnie M. Sarwal -- RNA Purification and Expression Analysis Using Microarrays and RNA Deep Sequencing / Steven R. Head [and others] |
| Summary |
After decades of research in clinical transplantation, new techniques have been developed that permit a further understanding of the immune mechanisms underlying immune recognition of allografts and a more accurate and thorough evaluation of compatibility between donors and recipients |
|
The second edition of Transplantation Immunology: Methods and Protocols expands upon the previous edition with current, detailed methods in transplantation immunology. The new methods chapters cover four major areas that are being applied in compatibility evaluations and ongoing transplantation immunology research |
|
Seven overview chapters provide reviews of the molecular basis for alloreactivity, current understanding of humoral and cellular mechanisms, as well as new developments in thoracic organ transplantation, composite tissue transplantation and in the transplantation of sensitized patients |
|
Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls |
|
Authoritative and practical, Transplantation Immunology: Methods and Protocol, Second Edition is devoted to transplantation immunology, both in the practice of compatibility testing and in transplantation research |
| Analysis |
chirurgie |
|
surgery |
|
transplantatie |
|
transplantation |
|
biomedische wetenschappen |
|
biomedicine |
|
immunologie |
|
immunology |
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Medicine (General) |
|
Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Transplantation immunology -- Laboratory manuals
|
|
Transplantation immunology.
|
|
Transplantation Immunology
|
|
Transplantation immunology
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Zachary, Andrea A
|
|
Leffell, Mary S
|
| ISBN |
9781627034937 |
|
1627034935 |
|
