| Description |
1 online resource (xvii, 231 pages, 9 unnumbered pages of plates) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 481 |
|
Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 481
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Springer protocols (Series)
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| Contents |
Human hepatocyte transplantation overview / J. Puppi and A. Dhawan -- Isolation of human hepatocytes / R.R. Mitry -- An optimised method for cryopreservation of human hepatocytes / C. Terry and R.D. Hughes -- Liver cell culture techniques / J.V. Castell and M.J. Gomez-Lechon -- In vitro assays for induction of drug metabolism / B.G. Lake [and others] -- Hepatocyte apoptosis / M. Najimi, F. Smets and E. Sokal -- Small animal models of hepatocyte transplantation / J. Seppen, E.E. Filali and R.O. Elferink -- Hepatocyte transplantation techniques: large animal models / A. Weber, M.T. Groyer-Picard and I. Dagher -- Cell transplant techniques: engraftment detection of cells / R.A. Fisher and V.R. Mas -- Hepatic preconditioning for transplanted cell engraftment and proliferation / Y.M. Wu and S. Gupta -- Ex vivo gene transfer into hepatocytes / X. Wang [and others] -- Sources of adult hepatic stem cells: haematopoietic / R. Jeffery, R. Poulsom and M.R. Alison -- Production of hepatocyte-like cells from human amnion / T. Miki [and others] -- Generation of hepatocytes from human embryonic stem cells / N. Safinia and S.L. Minger -- Isolation, in vitro cultivation and characterisation of foetal liver cells / Y. Wu, C.C. Shatapathy and S.L. Minger -- Human intrahepatic biliary epithelial cell lineages: studies in vitro / R. Joplin and S. Kachilele -- Liver cell labelling with MRI contrast agents / M. Modo, T.J. Meade and R.R. Mitry -- Microbiological monitoring of hepatocyte isolation in the GMP laboratory / S.C. Lehec |
| Summary |
Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Liver cells -- Transplantation
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Liver Diseases -- surgery
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Liver -- cytology
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Cell Transplantation
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Hépatocytes -- transplantation -- Cahiers de laboratoire.
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Séparation cellulaire -- méthodes -- Cahiers de laboratoire.
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Cellules hépatiques -- Transplantation.
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Dhawan, Anil
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|
Hughes, Robin D
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| ISBN |
9781597452014 |
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1597452017 |
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