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Title Plant signal transduction : methods and protocols / edited by Thomas Pfannschmidt
Published New York, NY : Humana ; London : Springer [distributor], ©2009

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Description 1 online resource (xi, 357 pages, 8 unnumbered pages of plates) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 479
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 479. 1064-3745
Springer protocols (Series)
Contents Multiple dimensions in plant signal transduction : an overview / Jorge J. Casal -- Phenotyping of Arabidopsis mutants for developmental effects of gene deletions / Cordelia Bolle -- Phenotyping of abiotic responses and hormone treatment in Arabidopsis / Cordelia Bolle -- Accurate real-time reverse transcription quantitative PCR / Marco Klatte and Petra Bauer -- Probing spatio-temporal intracellular calcium variations in plants / Axel Mithöfer, Christian Mazars, and Massimo E. Maffei -- Dynamic redox measurements with redox-sensitive GFP in plants by confocal laser scanning microscopy / Andreas J. Meyer and Thorsten Brach -- Imaging reactive oxygen species in vivo / Steven M. Driever [and others] -- Identification of thioredoxin targeted proteins using thioredoxin single cysteine mutant-immobilized resin / Ken Motohashi, Patrick G.N. Romano, and Toru Hisabori -- Determination of in vivo protein phosphorylation in photosynthetic membranes / Julia P. Vainonen, Alexander V. Vener, and Eva-Mari Aro -- Examining protein stability and its relevance for plant growth and development / Claus Schwechheimer [and others] -- Probing circadian rhythms in Chlamydomonas rheinhardtii by functional proteomics / Volker Wagner and Maria Mittag -- Bimolecular fluorescence complementation (BiFC) to study protein-protein interactions in living plant cells / Katia Schütze, Klaus Harter, and Christina Chaban -- Fluorescence cross-correlation spectroscopy of plant proteins / Hideki Muto, Masataka Kinjo, and Kotaro T. Yamamoto -- The determination of protein-protein interactions by the mating-based split-ubiquitin-system (mbSUS) / Christopher Grefen, Petr Obrdlik, and Klaus Harter -- Functional complementation of yeast mutants to study plant signalling pathways / Norbert Mehlmer, Elisabeth Scheikl-Pourkhalil, and Markus Teige -- Phosphatase activities analyzed by in vivo expressions / Alois Schweighofer, Zahra Ayatollahi, and Irute Meskiene -- Chromatin immunoprecipitation experiments to investigate in vivo binding of Arabidopsis transcription factors to target sequences / Benjamin Fode and Christiane Gatz -- Fluorescence-based electrophoretic mobility shift assay in the analysis of DNA-binding proteins / Sebastian Steiner and Thomas Pfannschmidt -- Analysis of plant regulatory DNA sequences by the yeast-one-hybrid assay / Dierk Wanke and Klaus Harter -- Analysis of plant regulatory DNA sequences by transient protoplast assays and computer aided sequence evaluation / Kenneth W. Berendzen, Klaus Harter, and Dierk Wanke -- RNA protein interaction mediating post-transcriptional regulation in the circadian system / Jan C. Schöning and Dorothee Staiger
Summary This text provides well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in plant analyses and the proteins involved in signal transduction
Bibliography Includes bibliographical references and index
Notes English
Subject Plant cellular signal transduction.
Plant molecular biology.
Plant physiology.
Cellular signal transduction.
Plant Development
Gene Expression Regulation, Plant
Plant Physiological Phenomena
Signal Transduction
Plant molecular biology.
Plants -- growth & development.
Gene Expression Regulation, Plant.
Plant Physiological Phenomena.
Signal Transduction.
Plant cellular signal transduction.
Plant physiology
Cellular signal transduction
Plant cellular signal transduction
Plant molecular biology
Transduction du signal.
Transduction du signal cellulaire chez les plantes.
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Pfannschmidt, Thomas.
ISBN 9781597452892
1597452890