| Description |
1 online resource (xxii, 303 pages) : illustrations |
| Series |
The practical approach series ; 201 |
|
Practical approach series ; 201.
|
| Contents |
DNA mobility shift assay / C.L. Dent, M.D. Smith, D.S. Latchman -- Footprint analysis of DNA : protein complexes in vitro and in vivo / Craig Spiro, Cynthia T. McMurray -- In vitro transcription and characterization of transcription / Austin J. Cooney, Sophia Y. Tsai, Ming-Jer Tsai -- Purification and cloning of DNA binding transcription factors / R.H. Nicolas, G. Hynes, G.H. Goodwin -- Cloning transcription factors from a cDNA expression library / Ian G. Cowell, Helen C. Hurst -- Cloning transcription factors by sequence similarity / Alan Ashworth -- Identification of target genes for a transcription factor by genomic binding-site cloning / Satoshi Inoue, Shigeru Kondo, Masami Muramatsu -- Analysis of cloned factors / Roger White, Malcolm Parker -- Neuronal promoter analysis by in vitro transcription using nuclear extracts from rat brain / M.L. Schwartz, W.W. Schlaepfer -- Preparation of chromatin templates for transcription studies / Joan Boyes -- Analysis of transcription factor modifications / N. Shaun B. Thomas |
| Summary |
"Since publication of the first edition of this book five years ago, a wide range of new methodologies have been developed to facilitate studies on transcription factors. The book has been radically updated and expanded so that it provides a comprehensive guide to the methods currently available to characterize the function and activity of an individual transcription factor. All the original chapters have either been fully updated or rewritten and additional chapters now included cover the use of in vitro transcription assays, analysis of the effect of transcription factors on chromatin structure, use of the genomic binding site assay and analysis of transcription factor modifications." "Each chapter includes full step-by-step protocols for immediate use at the laboratory bench and innumerable hints and tips for success. Anyone studying transcription factors in a research setting should find this book to be an invaluable bench companion."--Jacket |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Transcription factors -- Laboratory manuals
|
|
Genetic transcription -- Regulation -- Laboratory manuals
|
|
Transcription Factors -- physiology
|
|
SCIENCE -- Life Sciences -- Molecular Biology.
|
|
Genetic transcription -- Regulation
|
|
Transcription factors
|
|
Methode
|
|
Transkription Genetik
|
|
Transkriptionsfaktor
|
|
Facteurs de transcription -- Manuels de laboratoire.
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Latchman, David S
|
| ISBN |
0585483701 |
|
9780585483702 |
|
9780191565793 |
|
0191565792 |
|
