| Description |
1 online resource (xii, 270 pages) : illustrations (some color) |
| Series |
Methods in Molecular Biology ; v. 1890 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1890. 1064-3745
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| Contents |
Introduction to FOXO biology / Wolfgang Link -- Characterization of MicroRNAs regulating FOXO expression / Doug Hanniford and Eva Hernando -- Identification of transcription factor-binding sites in the mouse FOXO1 promoter / Christopher P. Cardozo -- Investigating mechanisms that control ubiquitin-mediated DAF-16/FOXO protein turnover / Thomas Heimbucher and Coleen T. Murphy -- Phosphorylation of FOXO proteins as a key mechanism to regulate their activity / Alba Orea-Soufi, David Davila, Maria Salazar-Roa, Maria de Mar Lorente, and Guillermo Velasco -- Methodological approach for the evaluation of FOXO as a positive regulator of antioxidant genes / Maria Monsalve, Ignacio Prieto, Andreza Fabro de Bem, and Yolanda Olmos -- Characterization of FOXO acetylation / Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-Garcia, and Eric W-F Lam -- Transcriptional activity of FOXO transcription factors measured by luciferase assays / Antonia Avila-Flores, Javier Arranz-Nicolas, and Isabel Merida -- Monitoring the transcriptional activity of FOXO transcription factors by analyzing their target genes / Vanessa Henriques, Susana Machado, Wolfgang Link, and Bibiana I. Ferreira -- Using ChIP-based approaches to characterize FOXO recruitment to its target promoters / Neeraj Kumar and Arnab Mukhopadhyay -- RNAi-mediated silencing of FOXO factors / Priyankar Sanphui, Akash Saha, and Subhas Chandra Biswas -- Immunofluorescence analysis by confocal microscopy for detecting endogenous FOXO / Francisco Castillo, Thomas A. Mackenzie, and Bastien Cautain -- High-throughput image-based screening to identify chemical compounds capable of activating FOXO / Marta Barradas, Wolfgang Link, Diego Megias, and Pablo J. Fernandez-Marcos -- Image-based identification of chemical compounds capable of trapping FOXO in the cell nucleus / Susana Machado, Catarina Raposo, Bibiana I. Ferreira, and Wolfgang Link -- Quantifying tissue-specific overexpression of FOXO in Drosophila via mRNA fluorescence in situ hybridization using branched DNA probe technology / Anna C. Blice-Baum, Georg Vogler, Meera C. Viswanathan, Bosco Trinh, Worawan B. Limpitikul, and Anthony Cammarato -- Genome-wide analysis for identifying FOXO protein-binding sites / Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, and Li Wang -- Mathematical modeling of nuclear trafficking of FOXO transcription factors / Bradford E. Peercy and Martin F. Schneider -- Following transcriptome to uncover FOXO biological functions / Raymond Liang, Vijay Menon, and Saghi Ghaffari -- Hydra as model to determine the role of FOXO in longevity / Thomas C.G. Bosch -- Genetic ablation of FOXO in mice to investigate its physiological role / Hwanhee Oh and Jihye Paik -- Analysis of FOXO3 gene polymorphisms associated with human longevity / Timothy A. Donlon, Philip M.C. Davy, and Bradley J. Willcox -- Analysis of FOXO3a gene polymorphism associated with asthma / Tejas Shah and Kalpana Joshi |
| Summary |
This volume provides an overview of experimental procedures and state-of art methods to investigate FOXOs. Chapters guide readers through biochemical and molecular methods, imaging approaches to monitor the subcellular localization of FOXO factors, omics and bioinformatics approaches, different animal models used in FOXO research, and human studies to investigate the role of FOXO factors in human disease and longevity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge FOXO Transcription Factors: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed November 12, 2018) |
| Subject |
Transcription factors -- Laboratory manuals
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Transcription factors.
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Transcription Factors
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Proteins.
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Science -- Life Sciences -- Biochemistry.
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Transcription factors
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| Genre/Form |
Electronic books
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| Form |
Electronic book
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Author |
Link, Wolfgang, editor
|
| ISBN |
9781493989003 |
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1493989006 |
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