| Description |
1 online resource (xii, 298 pages) : illustrations (some color) |
| Series |
Springer Protocols |
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Methods in molecular biology, 1940-6029 ; volume 1870 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 1870. 1064-3745
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| Contents |
Bisulfite sequencing of RNA for transcriptome-wide detection of 5-methylcytosine / Lukas Trixl, Dietmar Rieder, Thomas Amort, and Alexandra Lusser -- Single-molecule analysis of RNA dynamics in living cells using molecular beacons / Mingming Chen, Shiqi Mao, Xiaotian Wu, Zhao Ma, Yantao Yang, Christopher J. Krueger, and Antony K. Chen -- Visualization of Xist long noncoding RNA with a fluorescent CRISPR/Cas9 system / Urszula Wasþko, Zeming Zheng, and Sanchita Bhatnagar -- HAMR : high-throughput annotation of modified ribonucleotides / Lee E. Vandivier, Zachary D. Anderson, and Brian D. Gregory -- High-resolution mapping of N6-methyladenosine using m6A crosslinking immunoprecipitation sequencing (m6A-CLIP-Seq) / Phillip J. Hsu and Chuan He -- Direct chemical biotinylation of RNA 50-ends using a diazo reagent / Greggory M. Rice, Razvan Nutiu, and Christian M. Gampe -- Identification of methylated transcripts using the TRIBE approach |
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Lina Worpenberg, Tobias Jakobi, Christoph Dieterich, and Jean-Yves Roignant -- Decoding the atlas of RNA modifications from epitranscriptome sequencing data / Xiao-Qin Zhang and Jian-Hua Yang -- Detection of microRNA-mediated target mRNA cleavage and 30-uridylation in human cells by a SLA-RT-PCR analysis / Jing Lin and Lin Ji -- Genome-wide annotation of circRNAs and their alternative back-splicing/splicing with CIRCexplorer pipeline / Rui Dong, Xu-Kai Ma, Ling-Ling Chen, and Li Yang -- Synthesis and evaluation of novel neamine-nucleoside conjugates as potential antibiotic targets for Escherichia coli 16S ribosomal RNA / Zhen-Jun Yang, Maria-Grazia Concilio, Vasudevan Ramesh, and Li-He Zhang -- Electron microscope detection of 5-methylcytosine on DNA and RNA / Irene Masiello and Marco Biggiogera -- Immuno-northern blotting : detection of modified RNA using gel separation and antibodies to modified nucleosides / Eikan Mishima and Takaaki Abe |
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LncVar : deciphering genetic variations associated with long noncoding genes / Xiaowei Chen, Yajing Hao, Ya Cui, Zhen Fan, and Runsheng Chen -- Guided reconstruction of full-length isoforms from short reads by CIDANE / Sandro Andreotti and Stefan Canzar -- Profiling of N6-methyladenosine in zika virus RNA and host cellular mRNA / Gianluigi Lichinchi and Tariq M. Rana -- Detection and quantification of pseudouridine in RNA / Hironori Adachi, Meemanage D. DeZoysa, and Yi-Tao Yu -- 5-methylcytosine analysis by RNA-BisSeq / Yu-Sheng Chen, Hai-Li Ma, Ying Yang, Wei-Yi Lai, Bao-Fa Sun, and Yun-Gui Yang -- RNA modification regulatory genes in DNA damage / Radoslav Janostiak and Narendra Wajapeyee -- Dot blot analysis for measuring global N6-methyladenosine modification of RNA / Arvindhan Nagarajan, Radoslav Janostiak, and Narendra Wajapeyee -- Mapping and quantification of tRNA 20-O-methylation by RiboMethSeq |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed December 19, 2018) |
| Subject |
Transcription factors -- Laboratory manuals
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Transcription factors.
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Transcription Factors
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Transcriptome
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RNA-Binding Proteins
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Transcription factors
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| Form |
Electronic book
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Author |
Wajapeyee, Narendra, editor.
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Gupta, Romi, editor.
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| ISBN |
9781493988082 |
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1493988085 |
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