| Description |
1 online resource (xvi, 627 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; [v. 809] |
|
Methods in molecular biology (Clifton, N.J.) ; 809
|
| Contents |
Genome-wide in vivo cross-linking of sequence-specific transcription factors / Xiao-Yong li and Mark D. Biggin -- Characterization of complex regulatory networks and identification of promoter regulatory elements in yeast : "in silico" And "wet-lab" approaches / Nuno P. Mira, Miguel C. Teixeira, and Isabel Sá-Correia -- Electrophoretic mobility shift assay analysis of NF[kappa]B transcriptional regulation by nuclear I[kappa]B[alpha] / Ashish Juvekar [and others] -- Probing endogenous RNA polymerase II pre-initiation complexes by electrophoretic mobility shift assay / Emmanuelle Wilhelm, Christopher Takacs, and Brendan Bell -- Elucidating protein : DNA complex by oligonucleotide DNA affinity purification / Teddy T.C. Yang and Chi-Wing Chow -- Chromatin immunoprecipitation assay as a tool for analyzing transcription factor activity / Padmaja Gade and Dhan V. Kalvakolanu -- Two-step cross-linking for analysis of protein-chromatin interactions / Bing Tian, Jun Yan, and Allan R. Brasier -- Chromatin immunoprecipitation analysis of NF[kappa]B transcriptional regulation by nuclear I[kappa]B[alpha] in human macrophages / Sitharam Ramaswami [and others] -- In vivo ChIP for the analysis of microdissected tissue samples / Chris Murgatroyd, Anke Hoffmann, and Dietmar Spengler -- Quantification of protein-DNA interactions by in vivo chromatin immunoprecipitation in yeast / Amparo Pascual-Ahuir and Markus Proft -- Mapping protein-DNA interactions using ChIP-sequencing / Charles E. Massie and Ian G. Mils -- ChIP and re-ChIP assays : investigating interactions between regulatory proteins, histone modifications, and the DNA sequences to which they bind / Agnieszka D. Truax and Susanna F. Greer -- Transcriptional regulation of genes via hypoxia-inducible factor / Olga Roche and Michael Ohh -- Exchange protein directly activated by cyclic AMP-1-regulated recruitment of CCAAT/enhancer-binding proteins to the suppressor of cytokine signaling-3 promoter / William A. Sands [and others] -- Computational analysis of promoter elements and chromatin features in yeast / John J. Wyrick -- Chromatin affinity purification / Ryoko Harada and Alain Nepveu -- Determination of histone acetylation status by chromatin immunoprecipitation / Luciano Galdieri, John Moon, and Ales Vancura -- Immunostaining of Drosophila polytene chromosomes to investigate recruitment of chromatin-binding proteins / Magdalena Murawska and Alexander Brehm -- Detection of transcriptional activators, co-activators, and chromatin remodeling by chromatin immunoprecipitation coupled with real-time PCR / Tamara Y. Erkina and Alexandre M. Erkine -- Chromatin endogenous cleavage and psoralen crosslinking assays to analyze rRNA gene chromatin in vivo / Joachim Griesenbeck [and others] -- UV-induced DNA damage and DNA repair in ribosomal genes chromatin / Julie Pelloux [and others] -- Analysis of SUC2 promoter structure by nucleosome scanning / Jennifer Chang and Ales Vancura -- Chromatin immunoprecipitation of mouse embryos / Anne K. Voss [and others] -- Chromatin immunoprecipitation in mouse hippocampal cells and tissues / Badi Sri Sailaja, Takumi Takizawa, and Eran Meshorer -- Approaches for studying nucleosome movement by ATP-dependent chromatin remodeling complexes / Swetansu K. Hota and Blaine Bartholomew -- Mapping protein-DNA and protein-protein interactions of ATP-dependent chromatin remodelers / Swetansu K. Hota [and others] -- Evaluation of histone-modifying enzymes in stem cell populations / Leanne Stalker and Christopher Wynder -- Purification of multiprotein histone acetyltransferase complexes / Yuan-Liang Wang, Francesco Faiola, and Ernest Martinez -- Reconstitution of active and stoichiometric multisubunit lysine acetyltransferase complexes in insect cells / Kezhi Yan [and others] -- Affinity purification of MLL3/MLL4 histone H3K4 methyltransferase complex / Young-Wook Cho, SunHwa Hong, and Kai Ge -- Methods for analyzing histone citrullination in chromatin structure and gene regulation / Pingxin Li, Jing Hu, and Yanming Wang -- Analysis of mRNA abundance and stability by ribonuclease protection assay / Cristina Romero-López [and others] -- Array-based nuclear run-on analysis / Jinshui Fan [and others] -- In vivo run-on assays to monitor nascent precursor RNA transcripts / Piergiorgio Percipalle and Emilie Louvet -- Genome wide full-length transcript analysis using 5' and 3' paired-end-tag next generation sequencing (RNA-PET) / Xiaoan Ruan and Yijun Ruan -- Analysis of co-transcriptional RNA processing by RNA- ChIP assay / Danielle Bittencourt and Didier Auboeuf -- Quantitative analysis of transcription elongation by RNA polymerase I in vitro / David Alan Schneider -- Detection and characterization of transcription termination / Ghada Ghazal, Jules Gagnon, and Sherif Abou Elela -- Promoter-associated noncoding RNA from the CCND1 promoter / Xiaoyuan Song [and others] |
| Summary |
Through many recent remarkable developments, perhaps the most significant advancements in the study of transcriptional regulation are the development of genome-wide approaches for measuring gene expression, exemplified by gene chips (chip), and chromatin immunoprecipitation assays (ChIP) for measuring in vivo protein-DNA interactions at any genomic loci. Transcriptional Regulation: Methods and Protocols takes this progress and builds upon it with a collection of key protocols used in expert laboratories around the world. Divided into four convenient sections, this detailed volume explores promoter elements, transcription factors, and preinitiation complex (PIC) assembly, chromatin structure, chromatin modifying complexes, and RNA synthesis and regulation. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Comprehensive and accessible, Transcriptional Regulation: Methods and Protocols equally serves senior researchers and scientists experienced in transcriptional regulation as well as graduate students and scientists who wish to study transcriptional regulation for the first time |
| Analysis |
Life sciences |
|
genexpressie |
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gene expression |
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chromatine |
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chromatin |
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transcriptie |
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transcription |
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rna |
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Molecular Biology (General) |
|
Moleculaire biologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
"E-ISSN 1940-6029." |
| Subject |
Genetic transcription -- Regulation.
|
|
Genetic regulation.
|
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Genetic transcription.
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Gene Expression Regulation
|
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Transcription, Genetic
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Transcription Factors -- genetics
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Transcriptional Activation
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Genetic transcription
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Genetic regulation
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Genetic transcription -- Regulation
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Vancura, Ales.
|
| ISBN |
9781617793769 |
|
1617793760 |
|
