Limit search to available items
Book Cover

Title Transcription factor protocols / edited by Martin J. Tymms
Published Totowa, N.J. : Humana Press, ©2000


Description 1 online resource (x, 306 pages) : illustrations
Series Methods in molecular biology ; v. 130
Methods in molecular biology (Clifton, N.J.) ; v. 130
Contents Isolation of target gene promoter/enhancer sequences by whole genome PCR method -- In vivo footprinting using UV light and ligation-mediated PCR -- Identification of DNasel hypersensitive sites within nuclei -- Analysis of in vivo methylation -- Detection of transcription factor partners with a yease one hybrid screen -- Inverse PCR (IPCR) for obtaining promoter sequence -- PCR-directed linker scanning mutagenesis -- Transfection technologies -- The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues -- Optimizing electroporation conditions for the transformation of mammalian cells -- Calcium phosphate transfection of mammalian cultured cells -- DEAE-dextran transfection of mammaliam cultured cells -- Liposome-mediated transfection of mammaliam cells -- Assays for transcriptional activity based on the luciferase reporter gene -- Transient transfection of Schneider cells in the study of transcription factors -- Triplex-forming oligonucleotides and their use in the analysis of gene transcription -- Expression and purification of histidine-tagged transcription factors -- Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein -- Electrophoretic mobility shift assays -- In vitro promoter analysis using nuclear extracts and G-free cassette vectors -- In vitro transcription using competitor oligonucleotides to deplete specific transcription factors -- Computer software of eukaryotic promoter analysis
Summary In Transcription Factor Protocols, Martin Tymms has created a powerful compendium of the major techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. With chapters contributed by leading investigators well versed in the methods, this eminently practical compendium includes not only well established protocols, but also novel techniques that are now being widely adopted. Among the important new methods treated are the use of triplex-forming oligonucleotides, the application of whole genome PCR to the isolation of gene promoters/enhancers, the analysis of in vivo methylation, and in vivo footprinting using UV light and ligation-mediated PCR. Step-by-step instructions, tips about pitfalls to avoid, and extensive procedural notes all help to ensure robust and reproducible results. Transcription Factor Protocols offers both experienced workers and new researchers a vital first-stop reference collection of the core techniques for all those exploring the role of transcription factors in gene regulation today
Analysis transcriptiefactoren
transcription factors
moleculaire biologie
molecular biology
gene expression
polymerase chain reaction
biologische technieken
biological techniques
Molecular Biology (General)
Moleculaire biologie (algemeen)
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. MiAaHDL
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
In Springer Protocols
Subject Transcription factors -- Laboratory manuals
Transcription Factors -- analysis
Sequence Analysis, DNA -- methods
Transfection -- methods
SCIENCE -- Life Sciences -- Molecular Biology.
Transcription factors
Transfection -- méthodes.
Analyse de séquence d'ADN -- méthodes.
Facteurs de transcription -- analyse.
Wetenschappelijke technieken.
Séquençage des acides nucléiques.
Facteurs de transcription.
Genre/Form Handbooks and manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Tymms, Martin J.
ISBN 9781592596867