Title Genome editing and engineering : from TALENs, ZFNs and CRISPRs to molecular surgery / edited by Krishnarao Appasani ; with a foreword by George M. Church

Published Cambridge : Cambridge University Press, 2018 ©2018

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Description 1 online resource (xxix, 472 pages) : PDF file(s) Contents Cover -- Half-title page -- Title page -- Copyright page -- Dedication -- Contents -- List of Contributors -- Foreword -- Preface -- Part I: Biology of Endonucleases (Zinc-Finger Nuclease, TALENs and CRISPRs) and Regulatory Networks -- 1 Targeted Genome Editing Techniques in C. elegans and Other Nematode Species -- 2 Unbiased Detection of Off-target Cleavage by CRISPR/Cas9 and TALENs Using Integration-defective Lentiviral Vectors -- 3 In Vivo Studies of miRNA Target Interactions Using Site-specific Genome Engineering -- 4 Don't Kill the Messenger: Employing Genome Editing to Study Regulatory RNA Interactions -- Part II: Genome Editing in Model Organisms -- 5 Genome Editing in the Crustacean Daphnia magna using CRISPR/Cas and TALEN Systems -- 6 Leapfrogging: A Method for Targeting Genome Editing to the Germline -- 7 Genome Editing with Desired Mutations (Knockin) with CRISPR in Model Organisms -- 8 Genetically Engineered Pig Models for Human Diseases Using ZFNs, TALENs and CRISPR/Cas9 -- 9 Gene Editing to Create Agricultural and Biomedical Swine Models -- 10 Generation of New Model Cell Lines using ssODN Knockin Donors and FACS-based Genome Editing -- Part III: Technology Development and Screening -- 11 CRISPR Genome Editing in Mice -- 12 Detection of Insertion/Deletion (Indel) Events after Genome Targeting: Pros and Cons of the Available Methods -- Plates -- 13 Application of TAL Proteins and the CRISPR System to Purification of Specific Genomic Regions for Locus-specific Identification of Chromatin-associated Molecules -- 14 Application of CRISPR for Pooled, Vector-based Functional Genomic Screening in Mammalian Cell Lines -- 15 Generation and Utilization of CRISPR/Cas9 Screening Libraries in Mammalian Cells -- Part IV: Genome Editing in Stem Cells and Regenerative Biology -- 16 Targeted Genome Editing Using Nuclease-assisted Vector Integration 17 Genome Engineering Using Sleeping Beauty Transposition in Vertebrates -- 18 Genome Editing of Pluripotent Stem Cells -- 19 Principles for Targeting Adult Tissue Stem Cells to Achieve Durable Gene and Gene Editing Therapies -- 20 Therapeutic Genome Editing in Human Hematopoietic Stem and Progenitor Cells -- Part V: Genome Editing in Disease Biology -- 21 CRISPR/Cas9-based In Vivo Models of Cancer -- 22 Inducible CRISPR-based Genome Editing for the Characterization of Cancer Genes -- 23 Genome Editing for Retinal Diseases -- 24 Manipulation of Long Non-coding RNAs in Cardiovascular Disease Using Genome Editing Technology -- 25 Gene Silencing, Disruption and Latency Reactivation with RNA-based and Gene Editing CRISPR/Cas, ZFN and TALEN Technologies for HIV-1/AIDS Therapies -- 26 Use of the CRISPR/Cas9 System for Genome Editing of Immune System Cells, Defense Against HIV-1 and Cancer Therapies -- 27 Harnessing the Therapeutic Potential of Long Non-coding RNAs in Immunity -- Part VI: Legal (Intellectual Property) and Bioethical Issues of Genome Editing -- 28 Patenting of CRISPR-based Inventions -- 29 Patent, Ownership and Licensing Issues of CRISPR-based Genome Editing: Impact on Universities and their Licensees -- 30 The Ethics of Human Genome Editing -- 31 Regulating Human Genome Editing: Negotiating Ethical Concerns Through Science and Policy -- Index Summary Recent advances in genome editing tools using endonucleases such as TALENs, ZFNs, and CRISPRs, combined with genomic engineering technologies, have opened up a wide range of opportunities from applications in the basic sciences and disease biology research, to the potential for clinical applications and the development of new diagnostic tools. This complete guide to endonuclease-based genomic engineering gives readers a thorough understanding of this rapidly expanding field. Chapters cover the discovery, basic science, and application of these techniques, focusing particularly on their potential relevance to the treatment of cancer, and cardiovascular and immunological disease. The final section discusses the legal and ethical issues which accompany the technology. Providing authoritative coverage of the potential that genome editing and engineering have, this is an ideal reference for researchers and graduate students and those working in the biotechnology and pharmaceutical industries, as well as in a clinical setting Notes Title from publisher's bibliographic system (viewed on 01 Aug 2018) Bibliography Includes bibliographical references Notes Description based on online resource; title from digital title page (viewed on October 23, 2018) Subject Genetics -- Computer simulation Genetic engineering. Gene editing. Zinc-finger proteins. CRISPR (Genetics) Gene Editing Transcription Activator-Like Effector Nucleases Zinc Fingers Clustered Regularly Interspaced Short Palindromic Repeats Zinc-finger proteins Gene editing Genetic engineering Genetics -- Computer simulation Form Electronic book Author Appasani, Krishnarao, 1959- editor. ISBN 9781316756300 1316756300 1316621464 9781316621462

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