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Title In vitro transcription and translation protocols / edited by Martin J. Tymms
Published Totowa, N.J. : Humana Press, ©1995


Description 1 online resource (xii, 432 pages) : illustrations
Series Methods in molecular biology ; v. 37
Methods in molecular biology (Clifton, N.J.) ; v. 37
Contents Transcription in vitro using bacteriophage RNA polymerases / Elaine T. Schenborn -- Subtraction hybridization cDNA libraries / Clifford W. Schweinfest [and others] -- Quantitative measurement of mRNA using the RNase protection assay / Martin J. Tymms -- Assembly and transcription of chromatin templates using RNA polymerase III / Sara J. Felts -- Antisense affinity depletion of RNP particles : application to spliceosomal snRNPs / Benjamin J. Blencowe, Silvia M.L. Barabino -- In vitro mRNA editing using S100 extracts / Jobst Greeve, James Scott -- In vitro transcription assay for probing drug-DNA interactions during active transcription of DNA / Don R. Phillips, Donald M. Crothers -- In vitro reconstitution of progesterone-dependent RNA transcription in nuclear extracts of human breast carcinoma cells / Milan K. Bagchi, Sophia Y. Tsai, Ming-Jer Tsai -- Plastid in vitro transcription / Kai Tiller, Gerhard Link -- Microinjection of in vitro transcribed RNA and antisense oligonucleotides in mouse oocytes and early embryos to study the gain- and loss-of-function of genes / Ismail Kola, Sony Heru Sumarsono -- mRNA translation in Xenopus oocytes / Aldo Ceriotti, Alan Colman -- Xenopus egg extract translation system / Glenn M. Matthews, Alan Colman -- In vitro translation using rabbit reticulocyte lysate / Gregory S. Beckler, David Thompson, Tom Van Oosbree -- In vitro transcription system from BmN cells of the silkworm, Bombyx mori / Hiroshi Sakurai, Susumu Izumi, Shiro Tomino -- Translation using a wheat-germ extract / John F. Van Herwynen, Gregory S. Beckler -- In vitro transcription and translation in a cell-free system from Clostridium tetani / Bettina Andersen-Bechk, Heiner Niemann -- Preparation and use of E. coli S-30 extracts / Scott A. Lesley -- Posttranslational transport and in thylakoido integration of plastid protein / Elisabeth Kruse, Klaus Kloppstech -- Import into isolated yeast mitochondria of radiolabeled proteins synthesized in vitro / Ruby H.P. Law, Phillip Nagley -- Structure-function studies based on in vitro expression / Martin J. Tymms, Paul J. Hertzog -- [Delta]-like plastid transcription factors / Kai Tiller, Gerhard Link -- Preparation of HeLa nuclear extracts / B. Franklin Pugh -- Purification of the human TATA-binding protein, TBP / B. Franklin Pugh -- DNA-binding studies using in vitro synthesized Myb proteins / Robert George Ramsay -- DNase I footprinting using PCR-generated end-labeled DNA probes / Rocco C. Iannello -- Gene isolation by screenig [gamma]gt11 expression libraries with DNA binding-site probes / Bill Kalionis -- Transcriptional activation analysis by the chloramphenicol acetyl transferase (CAT) enzyme assay / David R. Hodge [and others]
Summary This book provides molecular biology laboratories with the most powerful techniques for employing in vitro transcription and translation systems. Detailed experimental protocols are provided for prokaryotic transcription and translation systems, along with protocols for the many techniques used in the analysis of eukaryotic transcription. The collection is unique in that it also contains protocols for core techniques that use the products of in vitro transcription and translation systems, such as protein import into mitochondria and chloroplasts and structure-function studies. Also covered are core techniques in the analysis of promoters and transcription factors
Analysis transcriptie
in vitro
gene expression
moleculaire biologie
molecular biology
biologische technieken
biological techniques
Molecular Biology (General)
Moleculaire biologie (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Molecular genetics -- Laboratory manuals
Genetic transcription -- Laboratory manuals
Genetic translation -- Laboratory manuals
Genetic transcription.
Genetics -- Technique.
Genetic translation.
Molecular biology.
Protein Biosynthesis
Transcription, Genetic
Genetic Techniques
Molecular Biology
molecular biology.
Molecular biology
Genetics -- Technique
Genetic transcription
Genetic translation
Molecular genetics
Transkription Genetik
Translation Genetik
Moleculaire biologie.
Transcriptie (biochemie)
Translatie (biochemie)
Wetenschappelijke technieken.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Tymms, Martin J.
ISBN 9781592595242