| Description |
1 online resource (xii, 312 pages) : illustrations |
| Series |
Springer protocols |
|
Methods in molecular biology, 1940-6029 ; 724 |
|
Springer protocols (Series)
|
|
Methods in molecular biology (Clifton, N.J.) ; v. 724. 1064-3745
|
| Contents |
Regulatory and ethical Issues on the utilization of FFPE tissues in research / Catherine M. With, David L. Evers, and Jeffrey T. Mason -- Tissue microarrays : construction and uses / Carol B. Fowler [and others] -- Standardization in immunohistology / Anthony S.-Y. Leong and Trishe Y.-M. Leong -- Multiple immunofluorescence labeling of formalin-fixed paraffin-embedded tissue / David Robertson and Clare M. Isacke -- Microwaves for chromogenic In situ hybridization / Anthony S.-Y. Leong and Zenobia Haffajee -- Automated analysis of FISH-stained HER2/neu samples with metafer / Christian Schunck and Eiman Mohammad -- Laser capture microdissection of FFPE tissue sections bridging the gap between microscopy and molecular analysis / Renate Burgemeister -- Nucleic acids extraction from laser microdissected FFPE tissue sections / Renate Burgemeister -- Microarray-based CGH and copy number analysis of FFPE samples / Fahd Al-Mulla -- Microarray profiling of DNA extracted from FFPE tissues using SNP 6.0 affymetrix platform / Marianne Tuefferd [and others] -- Whole genome amplification of DNA extracted from FFPE tissues / Mira Bosso and Fahd Al-Mulla -- Pyrosequencing of DNA extracted from formalin-fixed paraffin-embedded tissue / Brendan Doyle, Ciaran O'Riain, and Kim Appleton -- Analysis of DNA methylation in FFPE tissues using the methylight technology / Ashraf Dallol [and others] -- RT-PCR gene expression profiling of RNA from paraffin-embedded tissues prepared using a range of different fixatives and conditions / Mei-Lan Liu [and others] -- RT-PCR-based gene expression profiling for cancer biomarker discovery from fixed, paraffin-embedded tissues / Aaron Scott [and others] -- MicroRNA isolation from formalin-fixed, paraffin-embedded tissues / Aihua Liu and Xiaowei Xu -- Gene expression profiling of RNA extracted from FFPE tissues : NuGEN technologies' whole-transcriptome amplification system / Leah Turner, Joe Don Heath, and Nurith Kurn -- Protein mass spectrometry applications on FFPE tissue sections / Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason -- Alternative fixative to formalin fixation for molecular applications : the RCL2®-CS100 approach / Amelie Denouel [and others] |
| Summary |
Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research |
| Analysis |
geneeskunde |
|
medicine |
|
pathologie |
|
pathology |
|
biomedische wetenschappen |
|
biomedicine |
|
weefsels |
|
tissues |
|
Medicine (General) |
|
Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Tissues -- Analysis.
|
|
Tissue culture.
|
|
Diagnostic immunohistochemistry.
|
|
Tissue Culture Techniques
|
|
Tissue Array Analysis -- methods
|
|
Tissue Culture Techniques -- ethics
|
|
Paraffin Embedding -- methods
|
|
Diagnostic immunohistochemistry
|
|
Tissue culture
|
|
Tissues -- Analysis
|
|
Human Anatomy & Physiology.
|
|
Biology.
|
|
Health & Biological Sciences.
|
|
Physiology.
|
|
Biophysics.
|
| Form |
Electronic book
|
| Author |
Al-Mulla, Fahd.
|
| ISBN |
9781617790553 |
|
1617790559 |
|
