Title Cardiac gene expression : methods and protocols / edited by Jun Zhang and Gregg Rokosh

Published Totowa, N.J. : Humana Press, ©2007

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Description 1 online resource (xi, 365 pages) : illustrations Series Methods in molecular biology ; 366 Methods in molecular biology (Clifton, N.J.) ; v. 366 Contents Cardiac gene expression: the global perspective. Microarray analysis of gene expression in murine cardiac graft infiltrating cells / Yurong Liang, Xin Lu, and David L. Perkins. Expression profiling using Affymetrix GeneChip probe arrays / Martina Schinke-Braun and Jennifer A. Couget. Serial analysis of gene expression (SAGE): a useful tool to analyze the cardiac transcriptome / Kirill V. Tarasov [and others] Functional genomics by cDNA subtractive hybridization / Christophe Depre. Statistical methods in cardiac gene expression profiling: from image to function / Sek Won Kong -- Cardiac gene regulation: gene-specific mRNA measurement in the myocardium. Measurement of cardiac gene expression by reverse transcription polymerase chain reaction (RT-PCR) / Nicola King. Quantitative (real-time) RT-PCR in cardiovascular research / Kevin John Ashton and John Patrick Headrick. RNase protection assay for quantifying gene expression levels / Yongxia Qu and Mohamed Boutjdir. In situ hybridization: a technique to study localization of cardiac gene expression / Thierry P. Calmels and David Mazurais -- Cardiac gene regulation: promoter characterization in the myocardium. Characterization of cis-regulatory elements and transcription factor binding: gel mobility shift assay / Jim Jung-Ching Lin [and others] -- Mapping transcriptional start sites and in silico DNA footprinting / Martin E. Cullen and Paul J.R. Barton. Characterization of cardiac gene promoter activity: reporter constructs and heterologous promoter studies / Hsiao-Huei Chen and Alexandre F.R. Stewart -- In silico assessment of regulatory cis-elements and gene regulation. Comparative genomics: a tool to functionally annotate human DNA / Jan-Fang Cheng, James R. Priest, and Len A. Pennacchio. Developing computational resources in cardiac gene expression / Michael B. Bober and Raimond Winslow -- Cardiac single nucleotide polymorphisms. In silico analysis of SNPs and other high-throughput data / Neema Jamshidi, Thuy D. Vo, and Bernhard O. Palsson. Discovery and identification of sequence polymorphisms and mutations with MALDI-TOF MS / Dirk van den Boom and Mathias Ehrich -- Gene overexpression and targeting in the myocardium. Conditional targeting: inducible deletion by cre recombinase / Kelly R. O'Neal and Ramtin Agah. Cardiomyocyte preparation, culture, and gene transfer / Alexander H. Maass and Massimo Buvoli. Adeno-associated viral vector-delivered hypoxia-inducible gene expression in ischemic hearts / Hua Su and Yuet Wai Kan. Lentivirus-mediated gene expression / Jing Zhao and Andrew M.L. Lever Summary Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Myocardium -- Laboratory manuals Gene expression -- Laboratory manuals Genetic regulation -- Laboratory manuals Genetic regulation. Myocardium. Heart. Musculoskeletal system. Cardiovascular system. Anatomy. Genetics -- Technique. Muscles. Gene Expression Regulation Gene Expression Profiling Myocardium Heart Muscle, Striated Publication Formats Musculoskeletal System Cardiovascular System Investigative Techniques Publication Characteristics Genetic Phenomena Tissues Phenomena and Processes Anatomy Analytical, Diagnostic and Therapeutic Techniques and Equipment Genetic Techniques Muscles Animal Structures anatomy. MEDICAL -- Physiology. SCIENCE -- Life Sciences -- Human Anatomy & Physiology. Gene expression. Genetic regulation. Myocardium. Musculoskeletal system Muscles Heart Genetics -- Technique Cardiovascular system Anatomy Gene expression Genetic regulation Myocardium Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Zhang, Jun, 1968- Rokosh, Gregg. LC no. 2006008866 ISBN 1588293521 9781588293527 9781597450300 1597450308 1280971665 9781280971662 9786610971664 6610971668

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