Introduction. Electron Microscopy of Macromolecular Assemblies. Two-Dimensional Averaging Techniques. Multivariate Statistical Analysis and Classification of Images. Three-Dimensional Reconstruction. Interpretation of Three-Dimensional Images of Macromolecules. Example for an Apllication: Calcium Release Channel. Appendices
Summary
Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features * Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized * Describes methods that have been instrumental in exploring the three-dimensional structure of * the nuclear pore complex * the calcium release channel; * the ribosome * chaperonins
Analysis
Electron microscopy
Bibliography
Includes bibliographical references (pages 293-331) and index
