Contents; Foreword; I. PCR AND TECHNOLOGY TRANSFER; II. SELECTED PROTOCOLS; Appendix A: Construction of Laboratory Equipment; Appendix B: In-House Preparation of Reagents; Appendix C: Inventory for a PCR Laboratory; Appendix D: Good Laboratory Practice; Appendix E: Prevention of Cross-Contamination; Appendix F: PCR Troubleshooting Guide and Flowchart; Appendix G: Workshop Organization and Teaching Tips; Appendix H: Sample Charts and Worksheets; Appendix I: Useful World Wide Web Sites; Glossary; Afterword; Index
Summary
This text provides a description of the theoretical basis of polymerase chain reaction (PCR) and practical details of the method. It serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad