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Title Cytotoxic T-cells : methods and protocols / edited by Elena Ranieri
Published New York, NY : Humana Press, [2014]


Description 1 online resource (xii, 301 pages) : illustrations (some color)
Series Methods in Molecular Biology, 1940-6029 ; Volume 1186
Methods in molecular biology (Clifton, N.J.) ; v. 1186. 1064-3745
Contents 1. Negative and Positive Separation Techniques for the Isolation of Antigen-Specific CD8 T Cells from Blood and Tumor Tissue / Margherita Gigante, Sharon Natasha Cox, and Elena Ranieri -- 2. In Vitro\Ex Vivo Generation of Cytotoxic T Lymphocytes / Margherita Gigante and Elena Ranieri -- 3. Phenotypic and Functional Characterization of Cytotoxic T Lymphocytes by Flow Cytometry / Iulia Popescu, Matthew Pipeling, Jason Akulian, and John McDyer -- 4. Intracellular Signaling of CTLs / Sharon Natasha Cox -- 5. Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR / Devin B. Lowe, Jennifer L. Taylor, and Walter J. Storkus -- 6. CTL ELISPOT Assay / Elena Ranieri, Iulia Popescu, and Margherita Gigante -- 7. Methods of Purification of CTL-Derived Exosomes / Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morelli -- 8. Characterization of CTL by Microscopy / Giuseppe Stefano Netti -- 9. Intravital Imaging of Cytotoxic T Lymphocytes / Gaetano Faleo, Per-Olof Berggren, and Antonello Pileggi -- 10. The Microarray-Based Approach for the Analysis of the Transcriptome / Matteo Accetturo, Paola Pontrelli, and Loreto Gesualdo -- 11. miRNome Analysis Using Real-Time PCR / Paola Pontrelli, Matteo Accetturo, and Loreto Gesualdo -- 12. Proteomic Approaches by SELDI and MALDI-TOF/MS for CTL Analysis / Massimo Papale and Maria Teresa Rocchetti -- 13. Two-Dimensional Gel Electrophoresis Approach for CTL Phosphoproteome Analysis / Maria Teresa Rocchetti, Massimo Papale, and Loreto Gesualdo -- 14. Targeting the JAK/STAT Pathway in Cytotoxic T lymphocytes (CTL) by Next Generation Sequencing (NGS) / Maddalena Gigante, Sterpeta Diella, and Elena Ranieri -- 15. Experimental Model for Studying the Involvement of Regulatory Cytotoxic T Cells in Bone Resorption / Giacomina Brunetti, Angela Oranger, Silvia Colucci, and Maria Grano -- 16. CTL and Transplantation: Tissue In Vivo Characterization / Federica Rascio, Chiara Divella, and Giuseppe Grandaliano
Summary "In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls."--Publisher's description
Analysis biomedische wetenschappen
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed Sept. 4, 2014)
Subject T cells -- Laboratory manuals
Systems biology -- Laboratory manuals
T-Lymphocytes, Cytotoxic
Systems Biology -- methods
T cells
Systems biology
Biology -- Laboratory manuals
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Ranieri, Elena, editor
LC no. 2014944379
ISBN 9781493911585